| The ouabain-binding site of the α2 isoform of Na,K-ATPase plays a role in blood pressure regulation during pregnancy. | |
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MedLine Citation:
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PMID: 20940714 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The cardiotonic steroid/ouabain-binding site of the α subunit of Na,K-ATPase is thought to play an important role in cardiovascular homeostasis. Previously, we demonstrated the cardiotonic steroid-binding site of the α2 Na,K-ATPase is involved in adrenocorticotropic hormone (ACTH)-induced hypertension by using gene-modified α2(R/R) mice in which the cardiotonic steroid-binding site is relatively resistant to ouabain compared to the ouabain-sensitive wild-type α2(S/S) mice. To further explore the importance of this site in the cardiovascular system, we investigated blood pressure regulation during pregnancy in mice with the α2(R/R) isoform. METHODS: The systolic blood pressure (SBP) of the α2(S/S) and α2(R/R) mice was measured before and during pregnancy by tail-cuff. The expression of the α isoforms of Na, K-ATPase in various tissues and plasma endogenous ouabain contents were assessed prior to pregnancy as well as days 7 and 17 of gestation. RESULTS: The α2(S/S) mice showed a gradual decrease in the SBP during the first two trimesters, followed by an increase above the preconceptional level in the third trimester. However, the α2(R/R) mice exhibited a lower blood pressure in the third trimester. The cardiac expression of the α2 Na,K-ATPase in the α2(S/S) mice was significantly less than that of the α2(R/R) mice throughout the pregnancy. The plasma endogenous ouabain concentration significantly increased by twofold at day 17 of pregnancy in the α2(R/R) mice but not in the α2(S/S) mice. CONCLUSIONS: The cardiotonic steroid-binding site of the α2 Na,K-ATPase plays a role in maintaining normal SBP during pregnancy. |
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Authors:
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Naomi Oshiro; Iva Dostanic-Larson; Jon C Neumann; Jerry B Lingrel |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-09-09 |
Journal Detail:
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Title: American journal of hypertension Volume: 23 ISSN: 1941-7225 ISO Abbreviation: Am. J. Hypertens. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-16 Completed Date: 2011-03-03 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 8803676 Medline TA: Am J Hypertens Country: United States |
Other Details:
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Languages: eng Pagination: 1279-85 Citation Subset: IM |
Affiliation:
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Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding Sites Blood Pressure / drug effects, physiology* Brain / enzymology Female Kidney / enzymology Mice Myocardium / enzymology Ouabain / metabolism Pregnancy Sodium-Potassium-Exchanging ATPase / genetics, metabolism*, physiology Systole |
| Grant Support | |
ID/Acronym/Agency:
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R01 HL028573-27/HL/NHLBI NIH HHS; R01 HL28573/HL/NHLBI NIH HHS; R01 HL66062/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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630-60-4/Ouabain; EC 3.6.1.-/Atp1a2 protein, mouse; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase |
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