| Otoc1: a novel otoconin-90 ortholog required for otolith mineralization in zebrafish. | |
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MedLine Citation:
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PMID: 18000829 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Within the vestibular system of virtually all vertebrate species, gravity and linear acceleration are detected via coupling of calcified masses to the cilia of mechanosensory hair cells. The mammalian ear contains thousands of minute biomineralized particles called otoconia, whereas the inner ear of teleost fish contains three large ear stones called otoliths that serve a similar function. Otoconia and otoliths are composed of calcium carbonate crystals condensed on a core protein lattice. Otoconin-90 (Oc90) is the major matrix protein of mammalian and avian otoconia, while otolith matrix protein (OMP) is the most abundant matrix protein found in the otoliths of teleost fish. We have identified a novel gene, otoc1, which encodes the zebrafish ortholog of Oc90. Expression of otoc1 was detected in the ear between 15 hpf and 72 hpf, and was restricted primarily to the macula and the developing epithelial pillars of the semicircular canals. Expression of otoc1 was also detected in epiphysis, optic stalk, midbrain, diencephalon, flexural organ, and spinal cord. During embryogenesis, expression of otoc1 mRNA preceded the appearance of omp-1 transcripts. Knockdown of otoc1 mRNA translation with antisense morpholinos produced a variety of aberrant otolith phenotypes. Our results suggest that Otoc1 may serve to nucleate calcium carbonate mineralization of aragonitic otoliths. |
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Authors:
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Jessica A Petko; Bonny B Millimaki; Victor A Canfield; Bruce B Riley; Robert Levenson |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Developmental neurobiology Volume: 68 ISSN: 1932-8451 ISO Abbreviation: Dev Neurobiol Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-01-21 Completed Date: 2008-04-21 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 101300215 Medline TA: Dev Neurobiol Country: United States |
Other Details:
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Languages: eng Pagination: 209-22 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Penn State University College of Medicine, Hershey, PA 17033, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Brain / cytology, embryology, metabolism Calcium Carbonate / metabolism Down-Regulation / genetics Extracellular Matrix Proteins / genetics, isolation & purification, metabolism* Gene Expression Regulation, Developmental / genetics Minerals / metabolism Molecular Sequence Data Otolithic Membrane / cytology, embryology*, metabolism Phylogeny Protein Biosynthesis / genetics RNA, Antisense / pharmacology RNA, Messenger / analysis, genetics, metabolism Semicircular Canals / cytology, embryology, metabolism Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Vestibule, Labyrinth / cytology, embryology*, metabolism Zebrafish / embryology*, genetics, metabolism Zebrafish Proteins / genetics, isolation & purification, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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MH 068789/MH/NIMH NIH HHS; P50 MH068789-020001/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Extracellular Matrix Proteins; 0/Minerals; 0/Oc90 protein, mouse; 0/Otoc1 protein, zebrafish; 0/RNA, Antisense; 0/RNA, Messenger; 0/Zebrafish Proteins; 471-34-1/Calcium Carbonate |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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