| Osteopontin is involved in migration of eosinophils in asthma. | |
| | |
MedLine Citation:
|
PMID: 19400906 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Osteopontin (OPN) is an extracellular matrix protein with a wide range of functions, and is involved in various inflammatory diseases. However, the role of OPN in eosinophilic airway inflammation is unclear. OBJECTIVE: To elucidate the role of OPN in eosinophilic airway inflammation. METHODS: OPN protein levels in induced sputum from asthmatic patients and healthy controls were measured. Eosinophil migration assays were performed in the presence or absence of OPN, a blocking antibody (Ab) recognizing its integrin-binding domain (2K1) and an anti-integrin alpha 4 Ab (P1H4). In the mouse asthma model, the levels of eosinophilia were examined in bronchoalveolar lavage fluids (BALFs) from ovalbumin (OVA)-sensitized and -challenged mice with or without administration of an Ab (M5) corresponding to human 2K1. RESULTS: Levels of OPN in induced sputum were significantly higher in asthmatic patients when compared with healthy controls. In addition, levels of OPN were correlated with the percentage of sputum eosinophils. OPN induced significant migration of human eosinophils and this effect was inhibited by 2K1 and P1H4. M5 significantly attenuated OVA-induced eosinophilia in BALFs. CONCLUSION: These results indicate that OPN plays a role in the migration of eosinophils into the airways and may be involved in the pathogenesis of asthma. |
| | |
Authors:
|
A Takahashi; M Kurokawa; S Konno; K Ito; S Kon; S Ashino; T Nishimura; T Uede; N Hizawa; S-K Huang; M Nishimura |
Related Documents
:
|
17548626 - Coexpression of il-5 and eotaxin-2 in mice creates an eosinophil-dependent model of res... 6105956 - The lung mast cell: its physiology and potential relevance to defense of the lung. 4426696 - Studies on the mechanism of endogenous pyrogen production. ii. role of cell products in... 8006446 - Il-4 induces chemotaxis of blood eosinophils from atopic dermatitis patients, but not f... 12828866 - The hypothalamic-pituitary-adrenal axis and viral infection. 18020606 - The cpg motif: implications for clinical immunology. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-04-23 |
Journal Detail:
|
Title: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology Volume: 39 ISSN: 1365-2222 ISO Abbreviation: Clin. Exp. Allergy Publication Date: 2009 Aug |
Date Detail:
|
Created Date: 2009-07-23 Completed Date: 2009-12-17 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8906443 Medline TA: Clin Exp Allergy Country: England |
Other Details:
|
Languages: eng Pagination: 1152-9 Citation Subset: IM |
Affiliation:
|
First Department of Medicine, Hokkaido University School of Medicine, Sapporo, Hokkaido, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Animals Antibodies / immunology Antigen-Antibody Reactions Asthma / immunology*, pathology Bronchial Hyperreactivity / immunology Bronchoalveolar Lavage Fluid / immunology Cell Movement / immunology Cytokines / analysis Disease Models, Animal Eosinophils / immunology* Female Humans Lung Neoplasms / immunology*, pathology, surgery Male Mice Mice, Inbred BALB C Middle Aged Osteopontin / immunology* |
| Grant Support | |
ID/Acronym/Agency:
|
AI052468/AI/NIAID NIH HHS; AI073610/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies; 0/Cytokines; 106441-73-0/Osteopontin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pathogenesis of chronic urticaria.
Next Document: Thymic stromal lymphopoietin, OX40-ligand, and interleukin-25 in allergic responses.