Document Detail

Osteopontin expression in normal and hypobaric hypoxia-exposed rats.
MedLine Citation:
PMID:  18284657     Owner:  NLM     Status:  MEDLINE    
AIM: Experimental pulmonary hypertension induced in a hypobaric hypoxic environment (HHE) is characterized by structural remodelling of the heart and pulmonary arteries. Osteopontin (OPN) has emerged as a key factor in cardiovascular remodelling in response to pressure or volume overload. We studied the possible effects of HHE on the OPN synthesis system. METHODS: One hundred and forty-eight male Wistar rats were housed in a chamber with conditions equivalent of an altitude of 5500 m for up to 21 days. RESULTS: Plasma OPN protein level was found to be significantly decreased on day 0.5 of exposure to HHE, as was the level in the adrenal gland (which secreted highest levels of OPN protein). In the right ventricle of the heart (mRNA) and the lung (protein), OPN expression was found to be significantly increased only on day 1 and day 5, respectively, of exposure to HHE. By immunohistochemistry, the distribution and intensity of OPN protein in several organs were found to alter during exposure to HHE. However, these changes in OPN synthesis did not coincide with the moderate increase in pulmonary arterial pressure (PAP) (maximal mean PAP, 24.5 mmHg) during HHE. CONCLUSION: Pulmonary hypertension in HHE with conditions equivalent of an altitude of 5500 m may induce little or no OPN in heart and lung. Sustained induction may require a more severe PAP overload.
M Uenoyama; S Ogata; K Nakanishi; F Kanazawa; S Hiroi; S Tominaga; Y Kanatani; A Seo; T Matsui; S Suzuki
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Publication Detail:
Type:  Journal Article     Date:  2008-02-12
Journal Detail:
Title:  Acta physiologica (Oxford, England)     Volume:  193     ISSN:  1748-1716     ISO Abbreviation:  Acta Physiol (Oxf)     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-05     Completed Date:  2008-12-04     Revised Date:  2009-02-03    
Medline Journal Info:
Nlm Unique ID:  101262545     Medline TA:  Acta Physiol (Oxf)     Country:  England    
Other Details:
Languages:  eng     Pagination:  291-301     Citation Subset:  IM    
Division of Environmental Medicine, National Defense Medical College Research Institute, Tokorozawa, Japan.
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MeSH Terms
Anoxia / metabolism*,  physiopathology
Atmosphere Exposure Chambers
Blood Pressure
Body Weight
Gene Expression
Hypertension, Pulmonary / metabolism,  physiopathology
Osteopontin / biosynthesis*,  blood,  genetics
Pulmonary Artery / physiopathology
RNA, Messenger / genetics
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction / methods
Reg. No./Substance:
0/RNA, Messenger; 106441-73-0/Osteopontin

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