Document Detail

Osteopontin but not osteonectin favors the metastatic growth of pancreatic cancer cell lines.
MedLine Citation:
PMID:  20495387     Owner:  NLM     Status:  MEDLINE    
Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer-related death in western countries and among the malignancies with the worst prognosis. Osteonectin and osteopontin, two proteins of the extracellular matrix, have been found to be upregulated in PDAC. In the present study the expression of osteopontin mRNA as determined in a panel of 14 human pancreatic cancer cell lines was significantly related to the growth of these cell lines in the liver of nude rats (p = 0.001); whereas osteonectin showed a trend of being negatively related to pancreatic cancer cell growth in vivo (p = 0.10). In an in vitro co-culture model of human Suit2-007 and rat AsML PDAC cells with rat hepatocytes, a clearly increased expression of OPN mRNA was found in the tumor cells. In addition, both downregulation of osteopontin with specific antisense oligonucleotides and treatment with exogenous rh-osteonectin were associated with reduced cell proliferation. In accordance with the latter finding downregulation of osteonectin was coupled with increased proliferation. This evidence supports a protumorigenic role of osteopontin and points to an antitumorigenic role of osteonectin in PDAC.
Maria Zhivkova-Galunska; Hassan Adwan; Ergül Eyol; Jörg Kleeff; Armin Kolb; Frank Bergmann; Martin R Berger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-26
Journal Detail:
Title:  Cancer biology & therapy     Volume:  10     ISSN:  1555-8576     ISO Abbreviation:  Cancer Biol. Ther.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-10-08     Completed Date:  2011-04-19     Revised Date:  2013-02-05    
Medline Journal Info:
Nlm Unique ID:  101137842     Medline TA:  Cancer Biol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  54-64     Citation Subset:  IM    
Toxicology and Chemotherapy Unit, German Cancer Research Center, Heidelberg, Germany.
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MeSH Terms
Blotting, Western
Carcinoma, Pancreatic Ductal / genetics,  metabolism,  secondary*
Cell Proliferation
Coculture Techniques
Disease Progression
Gene Expression Regulation, Neoplastic / physiology*
Hepatocytes / metabolism,  pathology
Liver Neoplasms / genetics,  metabolism,  prevention & control,  secondary*
Oligonucleotides, Antisense / pharmacology
Osteonectin / genetics*
Osteopontin / genetics*
Pancreatic Neoplasms / genetics,  metabolism,  pathology*
RNA, Messenger / genetics
Rats, Nude
Recombinant Proteins
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Reg. No./Substance:
0/Oligonucleotides, Antisense; 0/Osteonectin; 0/RNA, Messenger; 0/Recombinant Proteins; 106441-73-0/Osteopontin
Comment In:
Cancer Biol Ther. 2010 Jul 1;10(1):65-7   [PMID:  20657168 ]

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