Document Detail

Osteogenic protein-1 enhances phenotypic expression in ROS 17/2.8 cells.
MedLine Citation:
PMID:  7491944     Owner:  NLM     Status:  MEDLINE    
Osteogenic protein-1 (OP-1) stimulates bone morphogenesis in vivo and modulates osteoblast growth and differentiation in vitro. Treatment of ROS 17/2.8 cells with OP-1 resulted in a time- and concentration-dependent inhibition of [3H]thymidine incorporation. In contrast, OP-1 treatment stimulated phenotypic differentiation in ROS 17/2.8 cells, as indicated by enhanced 1) alkaline phosphatase activity (4-fold); 2) alkaline phosphatase mRNA (5-fold); 3) parathyroid hormone receptor mRNA (2-fold), and 4) parathyroid hormone-stimulated adenosine 3',5'-cyclic monophosphate accumulation (2-fold). OP-1-induced changes in cell growth and gene expression were sensitive to cycloheximide and actinomycin D. Measurement of [3H]thymidine incorporation and alkaline phosphatase activity in situ revealed heterogeneity in the cellular responses to OP-1. Proliferating cells exhibited less alkaline phosphatase activity than nonproliferating cells, whereas cells expressing high levels of alkaline phosphatase incorporated little [3H]thymidine. Our data delineating the responses of mature differentiated osteoblasts to OP-1 suggest that potentiation of osteoblast differentiated function is an important component of bone morphogenesis in vivo.
A M Kitten; J C Lee; M S Olson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  269     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-01-04     Completed Date:  1996-01-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E918-26     Citation Subset:  IM    
Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284-7760, USA.
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MeSH Terms
Alkaline Phosphatase / genetics,  metabolism
Bone Morphogenetic Protein 7
Bone Morphogenetic Proteins*
Cell Differentiation / drug effects
Cell Division / drug effects
Cyclic AMP / metabolism
Cycloheximide / pharmacology
DNA / biosynthesis
Dactinomycin / pharmacology
Gene Expression / drug effects
Osteoblasts / cytology,  drug effects*,  physiology*
Parathyroid Hormone / pharmacology
Proteins / pharmacology*
RNA, Messenger / metabolism
Receptors, Parathyroid Hormone / genetics
Thymidine / metabolism
Transforming Growth Factor beta*
Reg. No./Substance:
0/Bmp7 protein, rat; 0/Bone Morphogenetic Protein 7; 0/Bone Morphogenetic Proteins; 0/Parathyroid Hormone; 0/Proteins; 0/RNA, Messenger; 0/Receptors, Parathyroid Hormone; 0/Transforming Growth Factor beta; 50-76-0/Dactinomycin; 50-89-5/Thymidine; 60-92-4/Cyclic AMP; 66-81-9/Cycloheximide; 9007-49-2/DNA; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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