Document Detail

Osteogenic properties of various HeLa cell lines and the BMP family genes expression.
MedLine Citation:
PMID:  12854351     Owner:  NLM     Status:  MEDLINE    
Heterotopic ossicles were induced in thigh muscles of immunosuppressed mice by implantation of suspension of several HeLa cell lines. These ossicles are the object of our research on osteoporosis. It was found that various HeLa cell lines differ in their potential to induce osteogenesis. In the previous paper we demonstrated the involvement of bone morphogenetic proteins (BMP-4 and BMP-6) secreted by HeLa cells in this phenomenon. In the present paper we try to find out the reason of the heterogeneity of various cell lines regarding the differences in their osteoinductive potencies. By the use of semiquantitative RT-PCR the differences in mRNA expression for several isoforms of BMP proteins in examined HeLa cell lines were found. The presence or absence of some of the BMP isoforms seems to be correlated with the quantity of heterotopically induced mineralised tissues. This was measured by weighing the deposited mineral after digestion of soft tissues surrounding the induced ossicles. This finding is supporting the thesis on high and uncontrolled heterogeneity of various HeLa cell lines used all over the world.
I E Kochanowska; K Wlodarski; A Wojtowicz; K Niemira; K Ostrowski
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of transplantation : quarterly of the Polish Transplantation Society     Volume:  7     ISSN:  1425-9524     ISO Abbreviation:  Ann. Transplant.     Publication Date:  2002  
Date Detail:
Created Date:  2003-07-11     Completed Date:  2004-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9802544     Medline TA:  Ann Transplant     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  58-62     Citation Subset:  IM    
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences, Wroclaw, Poland.
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MeSH Terms
Bone Morphogenetic Proteins / genetics*
Gene Expression*
Hela Cells / physiology*
Mice, Inbred BALB C
Multigene Family*
Osteogenesis / genetics,  physiology*
Protein Isoforms / genetics
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Bone Morphogenetic Proteins; 0/Protein Isoforms; 0/RNA, Messenger

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