Document Detail

Osteogenetic epithelial-mesenchymal cell interactions.
MedLine Citation:
PMID:  954314     Owner:  NLM     Status:  MEDLINE    
FL, HeLa and other non-murine epithelial cells are capable of inducing chondroosseous differentiation when brought into contact with mouse thigh mesenchymal cells. Immunosuppression as produced by cortisone or mouse antilymphocyte serum is required to prevent rejection and to allow growth of the foreign epithelial cells. Cartilage appears within 7 days and bone within 9 days following epithelial cell injection. Electron microscopic studies indicate that the induced cartilage and bone is normal in basic strucutre, and that calcification is mediated by extracellular matrix vesicles. Various forms of mechanical and chemical injury have consistently failed to stimulate cartilage or bone formation in the mouse thigh. Explantation of preosseous thigh tumors to mouse brain allows continued growth of human and mouse cells with development of cartilage or bone in 67 per cent of implants. Microscopy indicates cartilage and bone formation by many mesenchymal cells which are not in direct contact with epithelium. To account for this, two mechanisms of bone induction are envisioned: (1) short-range diffusion of inductive substance(s) and/or (2) transfer of the inductive stimulus by direct surface contact from epithelial cell to mesenchymal cell to mesenchymal cell and so on.
H C Anderson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical orthopaedics and related research     Volume:  -     ISSN:  0009-921X     ISO Abbreviation:  Clin. Orthop. Relat. Res.     Publication Date:  1976 Sep 
Date Detail:
Created Date:  1976-11-01     Completed Date:  1976-11-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0075674     Medline TA:  Clin Orthop Relat Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  211-23     Citation Subset:  IM    
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MeSH Terms
Amnion / physiology
Bone Regeneration
Bone and Bones / injuries
Brain / surgery
Cartilage / physiology
Cell Differentiation
Cell Division
Cell Line
Epithelial Cells*
Epithelium / physiology*,  transplantation
Extraembryonic Membranes
Hela Cells / physiology
Mesoderm / cytology,  physiology*
Muscles / physiology
Neoplasm Transplantation
Neoplasms / pathology
Transplantation, Autologous
Transplantation, Heterologous

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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