Document Detail


Osteocyte hypoxia: a novel mechanotransduction pathway.
MedLine Citation:
PMID:  10484347     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bone is a unique tissue in which to examine mechanotransduction due to its essential role in weight bearing. Within bone, the osteocyte is an ideal cellular mechanotransducer candidate. Because osteocytes reside distant from the blood supply, their metabolic needs are met by a combination of passive diffusion and enhanced diffusion, arising when the tissue is loaded during functional activity. Therefore, we hypothesized that depriving a bone of mechanical loading (and thus eliminating diffusion enhanced by loading) would rapidly induce osteocyte hypoxia. Using the avian ulna model of disuse osteopenia, we found that 24 h of unloading results in significant osteocyte hypoxia (8.4 +/- 1.8%) compared with control levels (1.1 +/- 0.5%; P = 0.03). Additionally, we present preliminary data suggesting that a brief loading regimen is sufficient to rescue osteocytes from this fate. The rapid onset of the observed osteocyte hypoxia, the inhibition of hypoxia by brief loading, and the cellular consequences of oxygen deprivation are suggestive of a novel mechanotransduction pathway with implications across organ systems.
Authors:
J S Dodd; J A Raleigh; T S Gross
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  277     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-10-28     Completed Date:  1999-10-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C598-602     Citation Subset:  IM; S    
Affiliation:
Department of Orthopaedic Surgery, University of Cincinnati, Cincinnati, Ohio 45267-0212, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomechanics
Cell Hypoxia / physiology*
Homeostasis
Male
Osteocytes / metabolism,  physiology*
Oxygen / metabolism
Signal Transduction / physiology
Time Factors
Turkeys
Ulna / physiology
Weight-Bearing / physiology
Grant Support
ID/Acronym/Agency:
AG-14881/AG/NIA NIH HHS; AR-45665/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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