Document Detail


Osteoconductive phosphoserine-modified poly({varepsilon}-lysine) dendrons: synthesis, titanium oxide surface functionalization and response of osteoblast-like cell lines.
MedLine Citation:
PMID:  23193106     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lack of direct bonding between the surface of an implant and the mineralized bony tissue is among the main causes of aseptic loosening in titanium-based implants. Surface etching and ceramic coatings have led to improved osteointegration, but their clinical performance is still limited either by partial bonding or by coating delamination. In this work, a solid-phase synthesis method has been optimized to produce poly(ε-lysine) dendrons, the outermost branching generation of which is functionalized by phosphoserine (PS), a known catalyst of the biomineralization process. The dendrons were deposited onto etched titanium oxide surfaces as a near-to-monolayer film able to induce the formation of a homogeneous calcium phosphate phase in a simulated body fluid over 3 days. The dendron films also stimulated MG63 and SAOS-2 osteoblast-like cells to proliferate at a rate significantly higher than etched titanium, with SAOS-2 also showing a higher degree of differentiation over 14 days. PS-tethered dendron films were not affected by various sterilization methods and UV treatment appeared to improve the cell substrate potential of these films, thus suggesting their potential as a surface functionalization method for bone implants.
Authors:
S T Meikle; G Bianchi; G Olivier; M Santin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the Royal Society, Interface / the Royal Society     Volume:  10     ISSN:  1742-5662     ISO Abbreviation:  J R Soc Interface     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2012-11-29     Completed Date:  2013-05-01     Revised Date:  2014-02-14    
Medline Journal Info:
Nlm Unique ID:  101217269     Medline TA:  J R Soc Interface     Country:  England    
Other Details:
Languages:  eng     Pagination:  20120765     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Cell Proliferation
Dendrimers / chemical synthesis*,  chemistry*
Osteoblasts / metabolism,  physiology*
Phosphoserine / chemistry*
Polylysine / chemistry*
Prostheses and Implants*
Solid-Phase Synthesis Techniques / methods
Surface Properties
Titanium / chemistry*
Chemical
Reg. No./Substance:
0/Dendrimers; 15FIX9V2JP/titanium dioxide; 17885-08-4/Phosphoserine; 25104-18-1/Polylysine; D1JT611TNE/Titanium
Comments/Corrections

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