| Osteoclasts express the B2 isoform of vacuolar H(+)-ATPase intracellularly and on their plasma membranes. | |
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MedLine Citation:
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PMID: 8772466 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Osteoclasts express high levels of vacuolar H(+)-ATPase (V-ATPase) in their ruffled membranes, driving the secretion of H+ required for normal bone resorption. Previous reports have suggested that the B subunit of the osteoclast V-ATPase differs from those expressed in kidney and other tissues. In this study, B subunit isoform-specific antibodies and cDNA probes were used to examine which B subunit isoform is expressed in osteoclasts and osteoclast-like cells. Immunoblotting and RNA hybridization analysis were used to demonstrate that cells from an osteoclast-rich mouse bone marrow culture model express the B2 but not the B1 subunit isoform. Immunocytochemical staining of murine osteoclasts generated in vitro and of native rat osteoclasts in bone sections showed that the B2 but not the B1 isoform was expressed at high levels and was polarized to the ruffled membrane. Human marrow cultures and monocyte-derived macrophages, used as models for osteoclasts, also expressed the B2 but not the B1 subunit isoform. These results indicate that V-ATPases containing the B2 subunit isoform mediate osteoclast bone resorption. |
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Authors:
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B S Lee; L S Holliday; B Ojikutu; I Krits; S L Gluck |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 270 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1996 Jan |
Date Detail:
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Created Date: 1996-12-19 Completed Date: 1996-12-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: C382-8 Citation Subset: IM |
Affiliation:
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Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Bone Marrow / enzymology Bone Marrow Cells Cell Membrane / enzymology Cells, Cultured Humans Immunoblotting Immunohistochemistry Intracellular Membranes / enzymology Isoenzymes / metabolism* Macrophages / enzymology Mice Molecular Sequence Data Monocytes / enzymology Nucleic Acid Hybridization Oligonucleotide Probes / genetics Osteoclasts / enzymology* Proton-Translocating ATPases / metabolism* RNA / genetics Vacuoles / enzymology* |
| Grant Support | |
ID/Acronym/Agency:
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AR-32087/AR/NIAMS NIH HHS; DK-09976/DK/NIDDK NIH HHS; DK-38848/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 0/Oligonucleotide Probes; 63231-63-0/RNA; EC 3.6.3.14/Proton-Translocating ATPases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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