| Osteoclastogenesis and osteoclastic resorption of tricalcium phosphate: effect of strontium and magnesium doping. | |
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MedLine Citation:
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PMID: 22566212 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bone substitute materials are required to support the remodeling process, which consists of osteoclastic resorption and osteoblastic synthesis. Osteoclasts, the bone-resorbing cells, generate from differentiation of hemopoietic mononuclear cells. In the present study, we have evaluated the effects of 1.0 wt % strontium (Sr) and 1.0 wt % magnesium (Mg) doping in beta-tricalcium phosphate (β-TCP) on the differentiation of mononuclear cells into osteoclast-like cells and its resorptive activity. In vitro osteoclast-like cell formation, adhesion, and resorption were studied using osteoclast precursor RAW 264.7 cell, supplemented with receptor activator of nuclear factor κβ ligand (RANKL). Osteoclast-like cell formation was noticed on pure and Sr-doped β-TCP samples at day 8, which was absent on Mg-doped β-TCP samples indicating decrease in initial osteoclast differentiation due to Mg doping. After 21 days of culture, osteoclast-like cell formation was evident on all samples with osteoclastic markers such as actin ring, multiple nuclei, and presence of vitronectin receptor α(v)β(3) integrin. After osteoclast differentiation, all substrates showed osteoclast-like cell-mediated degradation, however, significantly restricted for Mg-doped β-TCP samples. Our present results indicated that substrate chemistry controlled osteoclast differentiation and resorptive activity, which can be used in designing TCP-based resorbable bone substitutes with controlled degradation properties. |
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Authors:
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Mangal Roy; Susmita Bose |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-05-05 |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: 100 ISSN: 1552-4965 ISO Abbreviation: J Biomed Mater Res A Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-07-25 Completed Date: 2012-11-27 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
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Languages: eng Pagination: 2450-61 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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W M Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Substitutes / chemistry, metabolism* Calcium Phosphates / chemistry, metabolism* Cell Adhesion Cell Differentiation Cell Line Magnesium / chemistry, metabolism* Mice Osteoclasts / cytology*, metabolism RANK Ligand / metabolism Strontium / chemistry, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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N1H-R01-EB-007351/EB/NIBIB NIH HHS; R01 EB007351/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bone Substitutes; 0/Calcium Phosphates; 0/RANK Ligand; 0/beta-tricalcium phosphate; 7439-95-4/Magnesium; 7440-24-6/Strontium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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