Document Detail

Osteoblasts and MG-63 osteosarcoma cells behave differently when in contact with ProRoot MTA and White MTA.
MedLine Citation:
PMID:  12887386     Owner:  NLM     Status:  MEDLINE    
AIM: To test the hypothesis that MG-63 osteosarcoma cells and primary osteoblasts react differently to ProRoot trade mark MTA (mineral trioxide aggregate) and White MTA by: (i) investigating the attachment of primary osteoblasts and MG-63 osteosarcoma cells to ProRoot trade mark MTA and White MTA; and (ii) comparing the osteogenic behaviour of both cell lines in contact with these endodontic materials. METHODOLOGY: Primary osteoblasts were harvested from foetal rat calvaria by sequential digestion and MG-63 osteosarcoma cells were purchased. Cells were exposed to ProRoot trade mark MTA and White MTA prepared according to the manufacturer's instructions. All samples and controls were prepared in quadruplicate. After 6, 9 and 13 days exposure to MTA, the cells were fixed and prepared for SEM examination. In addition, both the cell types were grown to confluence and exposed to beta-glycerophosphate and dexamethasone to assess mineralized nodule formation as a function of osteogenic behaviour. RESULTS: The number of cells on the surface of the culture dish and on top of the materials increased in all samples throughout the 3 time periods, except for White MTA where no primary osteoblasts were visible on top of the material by the end of 13 days. After exposing cells to differentiation medium nodules were observed in cultures of primary osteoblasts, but not of MG-63 osteosarcoma cells. CONCLUSIONS: Under the conditions of this study, whilst primary osteoblasts initially bound to White MTA, they did not survive on the surface by the end of 13 days. Primary osteoblasts formed mineralized nodules when exposed to differentiation medium, whilst MG-63 cells did not form nodules. As MG-63 cells do not behave osteogenically by forming mineralized nodules, and primary osteoblasts are more sensitive than MG-63 osteosarcoma cells to White MTA in cell culture, primary osteoblasts are more appropriate than MG-63 cells for testing endodontic materials in cell culture.
A L Pérez; R Spears; J L Gutmann; L A Opperman
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International endodontic journal     Volume:  36     ISSN:  0143-2885     ISO Abbreviation:  Int Endod J     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-07-30     Completed Date:  2003-11-28     Revised Date:  2008-08-07    
Medline Journal Info:
Nlm Unique ID:  8004996     Medline TA:  Int Endod J     Country:  England    
Other Details:
Languages:  eng     Pagination:  564-70     Citation Subset:  D    
Department of Restorative Sciences, Baylor College of Dentistry, a member of the Texas A&M University System Health Science Center, Dallas, Texas 75246, USA.
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MeSH Terms
Aluminum Compounds / pharmacology*
Calcium Compounds / pharmacology*
Cell Adhesion
Drug Combinations
Materials Testing
Microscopy, Electron, Scanning
Osteoblasts / drug effects*
Osteogenesis / drug effects
Oxides / pharmacology*
Rats, Sprague-Dawley
Root Canal Filling Materials / pharmacology*
Silicates / pharmacology*
Tumor Cells, Cultured / drug effects
Reg. No./Substance:
0/Aluminum Compounds; 0/Calcium Compounds; 0/Drug Combinations; 0/Oxides; 0/Root Canal Filling Materials; 0/Silicates; 0/mineral trioxide aggregate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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