Document Detail


Osteoblast response to biomimetically altered titanium surfaces.
MedLine Citation:
PMID:  18595788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bioinert titanium (Ti) materials are generally encapsulated by fibrous tissue after implantation into the living body. To improve the bone-bonding ability of Ti implants, we activated commercially pure titanium (cpTi) by a simple chemical pre-treatment in HCl and NaOH. Subsequently, we exposed the treated samples to simulated body fluid (SBF) for 2 (TiCT) and 14 days (TiHCA), respectively, to mimic the early stages of bone bonding and to investigate the in vitro response of osteoblasts on thus altered biomimetic surfaces. Sample surfaces were characterized by scanning electron microscopy, energy-dispersive X-ray analysis, cross-sectional transmission electron microscopy analyses, Fourier transform infrared and Raman spectroscopy. It was shown that the efflorescence consisting of sodium titanate that is present on pre-treated cpTi surfaces transformed to calcium titanate after 2 days in SBF. After 14 days in SBF a homogeneous biomimetic apatite layer precipitated. Human osteoblasts (MG-63) revealed a well spread morphology on both functionalized Ti surfaces. On TiCT, the gene expression of the differentiation proteins alkaline phosphatase (ALP) and bone sialo protein was increased after 2 days. On both TiCT and TiHCA, the collagen I and ALP expression on the protein level was enhanced at 7 and 14 days. The TiCT and the TiHCA surfaces reveal the tendency to increase the differentiated cell function of MG-63 osteoblasts. Thus, chemical pre-treatment of titanium seems to be a promising method to generate osteoconductive surfaces.
Authors:
J Barbara Nebe; Lenka Müller; Frank Lüthen; Andrea Ewald; Claudia Bergemann; Egle Conforto; Frank A Müller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-11
Journal Detail:
Title:  Acta biomaterialia     Volume:  4     ISSN:  1878-7568     ISO Abbreviation:  -     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-22     Completed Date:  2008-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233144     Medline TA:  Acta Biomater     Country:  England    
Other Details:
Languages:  eng     Pagination:  1985-95     Citation Subset:  IM    
Affiliation:
University of Rostock, Department of Internal Medicine, Biomedical Research Centre, Cell Biology, Schillingallee 69, Biomedicum, D-18057 Rostock, Germany. barbara.nebe@med.uni-rostock.de
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism
Biocompatible Materials / chemistry*
Biomimetics*
Bone Substitutes / chemistry
Bone and Bones / metabolism
Cell Line, Tumor
Humans
Microscopy, Confocal
Microscopy, Electron
Osteoblasts / metabolism*
Oxides / chemistry
Spectroscopy, Fourier Transform Infrared
Spectrum Analysis, Raman / methods
Surface Properties
Titanium / chemistry*
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Bone Substitutes; 0/Oxides; 12034-34-3/sodium titanate; 7440-32-6/Titanium; EC 3.1.3.1/Alkaline Phosphatase

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