| Osteoblast cell response to beta-tricalcium phosphate scaffolds with controlled architecture in flow perfusion culture system. | |
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MedLine Citation:
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PMID: 18283535 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Porous beta-tricalcium phosphate (beta-TCP) scaffolds with controlled architecture and improved mechanical properties were fabricated by combining the gel-casting and rapid prototyping techniques. The pore morphology, size, and distribution of the beta-TCP scaffolds were characterized using a scanning electron microscope. The porosity of the resulting scaffolds with pore size range from 300 to 500 microm was 46%. The average compressive strength was 16.1 MPa. X-ray diffraction was used to determine the crystal structure and chemical composition of scaffolds. The result indicated that the sintering process has not changed the composition of beta-TCP. Flow perfusion culture system was developed in our lab to improve mass transfer for seeded cells. For scaffold/cell constructs cultured under flow perfusion for 4, 8, and 16 days, there was greater scaffold cellularity and alkaline phosphatase activity compared with static culture condition. These results indicated that flow perfusion culture system had evident effects on osteoblast viability and functions in vitro. |
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Authors:
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Xiang Li; Dichen Li; Lin Wang; Bingheng Lu; Zhen Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-02-19 |
Journal Detail:
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Title: Journal of materials science. Materials in medicine Volume: 19 ISSN: 1573-4838 ISO Abbreviation: J Mater Sci Mater Med Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-05-13 Completed Date: 2009-05-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9013087 Medline TA: J Mater Sci Mater Med Country: United States |
Other Details:
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Languages: eng Pagination: 2691-7 Citation Subset: IM |
Affiliation:
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Xi'an Jiaotong University, China. Xiangliwj@yahoo.com.cn |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biocompatible Materials
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chemistry* Calcium Phosphates / chemistry* Cell Culture Techniques / methods* Cell Proliferation Cell Survival Flow Cytometry / methods* Materials Testing Mechanotransduction, Cellular / physiology* Osteoblasts / cytology, physiology* Osteogenesis / physiology* Perfusion / methods* |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Calcium Phosphates; 0/beta-tricalcium phosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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