Document Detail


Osteoarthritis synovial fluid activates pro-inflammatory cytokines in primary human chondrocytes.
MedLine Citation:
PMID:  23212731     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Two of the most common joint diseases are rheumatoid arthritis (RA) and osteoarthritis (OA). Cartilage degradation and erosions are important pathogenetic mechanisms in both joint diseases and have presently gained increasing interest. The aim of the present study was to investigate the effects of the synovial fluid environment of OA patients in comparison with synovial fluids of RA patients on human chondrocytes in vitro.
METHODS: Primary human chondrocytes were incubated in synovial fluids gained from patients with OA or RA. The detection of vital cell numbers was determined by histology and by using the Casy Cell Counter System. Cytokine and chemokine secretion was determined by a multiplex suspension array.
RESULTS: Microscopic analysis showed altered cell morphology and cell shrinkage following incubation with synovial fluid of RA patients. Detection of vital cells showed a highly significant decrease of vital chondrocyte when treated with RA synovial fluids in comparison with OA synovial fluids. An active secretion of cytokines such as vascular endothelial growth factor (VEGF) of chondrocytes treated with OA synovial fluids was observed.
CONCLUSIONS: Significantly increased levels of various cytokines in synovial fluids of RA, and surprisingly of OA, patients were shown. Activation of pro-inflammatory cytokines of human chondrocytes by synovial fluids of OA patient supports a pro-inflammatory process in the pathogenesis of OA.
Authors:
Paula Hoff; Frank Buttgereit; Gerd-Rüdiger Burmester; Manuela Jakstadt; Timo Gaber; Kristin Andreas; Georg Matziolis; Carsten Perka; Eric Röhner
Publication Detail:
Type:  Journal Article     Date:  2012-12-05
Journal Detail:
Title:  International orthopaedics     Volume:  37     ISSN:  1432-5195     ISO Abbreviation:  Int Orthop     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-31     Completed Date:  2013-05-27     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  7705431     Medline TA:  Int Orthop     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  145-51     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Arthritis, Rheumatoid / metabolism*
Cells, Cultured
Chemokine CCL2 / metabolism
Chondrocytes / chemistry,  metabolism*
Cytokines / metabolism*
Granulocyte Colony-Stimulating Factor / metabolism
Humans
Interferon-gamma / metabolism
Interleukin-6 / metabolism
Interleukin-8 / metabolism
Osteoarthritis / metabolism*
Statistics, Nonparametric
Synovial Fluid / chemistry*
Vascular Endothelial Growth Factor A / metabolism
Chemical
Reg. No./Substance:
0/Chemokine CCL2; 0/Cytokines; 0/Interleukin-6; 0/Interleukin-8; 0/Vascular Endothelial Growth Factor A; 143011-72-7/Granulocyte Colony-Stimulating Factor; 82115-62-6/Interferon-gamma
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