| OspE2 of Shigella sonnei is required for the maintenance of cell architecture of bacterium-infected cells. | |
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MedLine Citation:
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PMID: 16622194 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The OspE2 product of Shigella spp., the expression of which is regulated by the mxiE gene, is secreted through a type III secretion system into host cells. We investigated the function of OspE2 of Shigella sonnei by using cultured epithelial cells. Cells invaded by an ospE2 deletion mutant altered their morphology into the rounding shape, which was not due to cell death, whereas cells invaded by the wild-type strain kept their cell shape intact. The ospE2 mutation did not affect initial cell entry and multiplication in cells, but the mutant formed smaller-than-normal plaques on cell monolayers, indicating a deficiency in cell-to-cell spread by the bacteria. An mxiE deletion mutant also showed changes in cell morphology and deficiency in bacterial spread to adjacent cells. In cells invaded by the ospE2 mutant, disturbance of actin stress fibers was prominent at 3 h after invasion. Analysis of OspE2 localization indicated that the OspE2 protein accumulated on focal contact-like structures in the infected host cells. These results suggest that colocalization of the OspE2 protein in the focal contacts of infected cells may function to maintain an intact cell morphology. The morphological change induced by invasion of the ospE2 mutant may affect secondary bacterial transmission. |
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Authors:
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Masashi Miura; Jun Terajima; Hidemasa Izumiya; Jiro Mitobe; Teruya Komano; Haruo Watanabe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Infection and immunity Volume: 74 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-04-19 Completed Date: 2006-05-23 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 2587-95 Citation Subset: IM |
Affiliation:
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Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB185406; AB185407 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Apoptosis Bacterial Outer Membrane Proteins / genetics, physiology* Hela Cells Humans In Situ Nick-End Labeling Molecular Sequence Data Mutation Shigella sonnei / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Outer Membrane Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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