| Osmotically relevant membrane signaling complex: association between HB-EGF, beta(1)-integrin, and CD9 in mTAL. | |
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MedLine Citation:
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PMID: 10898725 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The integral membrane proteins cluster of differentiation-9 (CD9), beta(1)-integrin, and heparin-binding epidermal growth factor-like (HB-EGF) exist in association in many cell lines and are linked to intracellular signaling mechanisms. Two of the proteins (CD9 and beta(1)-integrin) are induced by hypertonicity, suggesting that their related signaling processes may be relevant to osmotic stress. The validity of this hypothesis rests upon coexpression and physical association between these molecules in nephron segments that are normally exposed to high and variable ambient osmolality. In this work, we show that CD9 and beta(1)-integrin are induced in rat kidney medulla after dehydration. Immunohistochemistry and immunoprecipitation studies show that CD9, HB-EGF, and beta(1)-integrin are coexpressed and physically associated in medullary thick ascending limbs (mTAL), nephron segments that are normally exposed to high and variable extracellular osmolality. Our findings are consistent with the existence of a cluster of integral membrane proteins in mTAL that may initiate or modulate osmotically relevant signaling pathways. |
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Authors:
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D Sheikh-Hamad; K Youker; L D Truong; S Nielsen; M L Entman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of physiology. Cell physiology Volume: 279 ISSN: 0363-6143 ISO Abbreviation: Am. J. Physiol., Cell Physiol. Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-08-17 Completed Date: 2000-08-17 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100901225 Medline TA: Am J Physiol Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: C136-46 Citation Subset: IM |
Affiliation:
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Renal Section, Baylor College of Medicine, Houston, Texas 77030, USA. sheikh@bcm.tmc.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD / metabolism* Antigens, CD29 / metabolism* Body Water / metabolism Epidermal Growth Factor / metabolism* Intercellular Signaling Peptides and Proteins Kidney Medulla / metabolism Loop of Henle / metabolism* Male Membrane Glycoproteins* Membranes / physiology Osmotic Pressure Rats Rats, Sprague-Dawley Signal Transduction / physiology* Tissue Distribution |
| Grant Support | |
ID/Acronym/Agency:
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1 RO1 DK-55137-01/DK/NIDDK NIH HHS; HL-42550/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, CD29; 0/CD9 antigen; 0/Intercellular Signaling Peptides and Proteins; 0/Membrane Glycoproteins; 149176-25-0/heparin-binding EGF-like growth factor; 62229-50-9/Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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