Document Detail


Osmotically induced cell swelling versus cell shrinking elicits specific changes in phospholipid signals in tobacco pollen tubes.
MedLine Citation:
PMID:  14739344     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pollen tube cell volume changes rapidly in response to perturbation of the extracellular osmotic potential. This report shows that specific phospholipid signals are differentially stimulated or attenuated during osmotic perturbations. Hypo-osmotic stress induces rapid increases in phosphatidic acid (PA). This response occurs starting at the addition of 25% (v/v) water to the pollen tube cultures and peaks at 100% (v/v) water. Increased levels of PA were detected within 30 s and reached maximum by 15 to 30 min after treatment. The pollen tube apical region undergoes a 46% increase in cell volume after addition of 100% water (v/v), and there is an average 7-fold increase in PA. This PA increase appears to be generated by phospholipase D because concurrent transphosphatidylation of n-butanol results in an average 8-fold increase in phosphatidylbutanol. Hypo-osmotic stress also induces an average 2-fold decrease in phosphatidylinositol phosphate; however, there are no detectable changes in the levels of phosphatidylinositol bisphosphates. In contrast, salt-induced hyperosmotic stress from 50 to 400 mm NaCl inhibits phospholipase D activity, reduces the levels of PA, and induces increases in the levels of phosphatidylinositol bisphosphate isomers. The pollen tube apical region undergoes a 41% decrease in cell volume at 400 mm NaCl, and there is an average 2-fold increase in phosphatidylinositol 3,5-bisphosphate and 1.4-fold increase in phosphatidylinositol 4,5-bisphosphate. The phosphatidylinositol 3,5-bisphosphate increase is detected within 30 s and reaches maximum by 15 to 30 min after treatment. In summary, these results demonstrate that hypo-osmotic versus hyperosmotic perturbation and the resultant cell swelling or shrinking differentially activate specific phospholipid signaling pathways in tobacco (Nicotiana tabacum) pollen tubes.
Authors:
Laura Zonia; Teun Munnik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-01-22
Journal Detail:
Title:  Plant physiology     Volume:  134     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-02-17     Completed Date:  2004-06-10     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  813-23     Citation Subset:  IM    
Affiliation:
Institute of Experimental Botany, Na Pernikarce 15, 160 00 Prague 6, Czech Republic. zonia@ueb.cas.cz
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MeSH Terms
Descriptor/Qualifier:
Flowers / growth & development*,  metabolism
Osmotic Pressure / drug effects
Phosphatidic Acids / biosynthesis
Phosphatidylinositol 4,5-Diphosphate / biosynthesis
Phosphatidylinositol Phosphates / biosynthesis
Phospholipase D / drug effects,  metabolism
Phospholipids / metabolism*
Signal Transduction / drug effects,  physiology*
Sodium Chloride / pharmacology
Tobacco / growth & development*,  metabolism
Water / pharmacology
Chemical
Reg. No./Substance:
0/Phosphatidic Acids; 0/Phosphatidylinositol 4,5-Diphosphate; 0/Phosphatidylinositol Phosphates; 0/Phospholipids; 0/phosphatidylinositol 3,5-diphosphate; 7647-14-5/Sodium Chloride; 7732-18-5/Water; EC 3.1.4.4/Phospholipase D
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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