| Osmotically induced cell swelling versus cell shrinking elicits specific changes in phospholipid signals in tobacco pollen tubes. | |
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MedLine Citation:
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PMID: 14739344 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pollen tube cell volume changes rapidly in response to perturbation of the extracellular osmotic potential. This report shows that specific phospholipid signals are differentially stimulated or attenuated during osmotic perturbations. Hypo-osmotic stress induces rapid increases in phosphatidic acid (PA). This response occurs starting at the addition of 25% (v/v) water to the pollen tube cultures and peaks at 100% (v/v) water. Increased levels of PA were detected within 30 s and reached maximum by 15 to 30 min after treatment. The pollen tube apical region undergoes a 46% increase in cell volume after addition of 100% water (v/v), and there is an average 7-fold increase in PA. This PA increase appears to be generated by phospholipase D because concurrent transphosphatidylation of n-butanol results in an average 8-fold increase in phosphatidylbutanol. Hypo-osmotic stress also induces an average 2-fold decrease in phosphatidylinositol phosphate; however, there are no detectable changes in the levels of phosphatidylinositol bisphosphates. In contrast, salt-induced hyperosmotic stress from 50 to 400 mm NaCl inhibits phospholipase D activity, reduces the levels of PA, and induces increases in the levels of phosphatidylinositol bisphosphate isomers. The pollen tube apical region undergoes a 41% decrease in cell volume at 400 mm NaCl, and there is an average 2-fold increase in phosphatidylinositol 3,5-bisphosphate and 1.4-fold increase in phosphatidylinositol 4,5-bisphosphate. The phosphatidylinositol 3,5-bisphosphate increase is detected within 30 s and reaches maximum by 15 to 30 min after treatment. In summary, these results demonstrate that hypo-osmotic versus hyperosmotic perturbation and the resultant cell swelling or shrinking differentially activate specific phospholipid signaling pathways in tobacco (Nicotiana tabacum) pollen tubes. |
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Authors:
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Laura Zonia; Teun Munnik |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-01-22 |
Journal Detail:
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Title: Plant physiology Volume: 134 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 2004 Feb |
Date Detail:
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Created Date: 2004-02-17 Completed Date: 2004-06-10 Revised Date: 2013-06-09 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 813-23 Citation Subset: IM |
Affiliation:
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Institute of Experimental Botany, Na Pernikarce 15, 160 00 Prague 6, Czech Republic. zonia@ueb.cas.cz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Flowers
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growth & development*,
metabolism Osmotic Pressure / drug effects Phosphatidic Acids / biosynthesis Phosphatidylinositol 4,5-Diphosphate / biosynthesis Phosphatidylinositol Phosphates / biosynthesis Phospholipase D / drug effects, metabolism Phospholipids / metabolism* Signal Transduction / drug effects, physiology* Sodium Chloride / pharmacology Tobacco / growth & development*, metabolism Water / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Phosphatidic Acids; 0/Phosphatidylinositol 4,5-Diphosphate; 0/Phosphatidylinositol Phosphates; 0/Phospholipids; 0/phosphatidylinositol 3,5-diphosphate; 7647-14-5/Sodium Chloride; 7732-18-5/Water; EC 3.1.4.4/Phospholipase D |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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