| Osmotic tolerance limits and effects of cryoprotectants on the motility, plasma membrane integrity and acrosomal integrity of rat sperm. | |
| | |
MedLine Citation:
|
PMID: 17084388 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Osmotic stress is an important factor that can result in cell damage during cryopreservation. The objectives of this study were to determine: (1) isosmotic sperm cell volume; (2) osmotically inactive volume; (3) osmotic tolerance limits of rat sperm; and (4) the effects of addition and removal of glycerol (Gly), ethylene glycol (EG), propylene glycol (PG) or dimethyl sulfoxide (Me(2)SO) on rat sperm function. Sperm from Fischer 344 and Sprague-Dawley rats were used in this study. An electronic particle counter was used to measure the cell volume of rat sperm. Computer-assisted sperm motility analysis and flow-cytometric analysis were used to assess sperm motility, plasma membrane and acrosomal integrity. The isosmotic sperm cell volumes of the two strains were 37.0+/-0.1 and 36.2+/-0.2 microm(3), respectively. Rat sperm behaved as linear osmometers from 260 to 450 mOsm, and the osmotically inactive sperm volumes of the two strains were 79.8+/-1.5% and 81.4+/-2.2%, respectively. Rat sperm have very limited osmotic tolerances. The sperm motility and the sperm plasma membranes of both strains were sensitive to anisosmotic treatments, but the acrosomes of both strains were more sensitive to hyposmotic than hyperosmotic conditions. The one-step addition and removal of Me(2)SO showed the most deleterious effect on rat sperm motility, plasma membrane integrity, and acrosomal integrity among the four cryoprotectants. These data characterizing rat sperm osmotic behavior, osmotic and cryoprotectant tolerance will be used to design cryopreservation protocols for rat sperm. |
| | |
Authors:
|
Wei Si; James D Benson; Hongsheng Men; John K Critser |
Related Documents
:
|
21887848 - Age-dependent expression of forkhead box o proteins in the duodenum of rats. 943528 - Reproduction in rats and mice isoimmunized with homogenates of ovary or testis with epi... 21845008 - Effect of unex on ethylene glycol-induced urolithiasis in rats. 19440488 - Time- and dose-related effects of di-(2-ethylhexyl) phthalate and its main metabolites ... 7056998 - Life span study of spf fischer 344 male rats fed ad libitum or restricted diets: longev... 907478 - Experimental infection of adult axenic rats with parker's rat coronavirus. |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural Date: 2006-11-03 |
Journal Detail:
|
Title: Cryobiology Volume: 53 ISSN: 0011-2240 ISO Abbreviation: Cryobiology Publication Date: 2006 Dec |
Date Detail:
|
Created Date: 2007-01-08 Completed Date: 2007-03-07 Revised Date: 2007-12-03 |
Medline Journal Info:
|
Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: United States |
Other Details:
|
Languages: eng Pagination: 336-48 Citation Subset: IM |
Affiliation:
|
Comparative Medicine Center, Research Animal Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, 1600 East Rollins Street, Columbia, MO 65211, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acrosome
/
drug effects,
physiology Animals Cell Membrane / drug effects, physiology Cryopreservation / methods* Cryoprotective Agents / pharmacology* Dimethyl Sulfoxide / pharmacology Ethylene Glycol / pharmacology Glycerol / pharmacology Male Osmotic Pressure Propylene Glycol / pharmacology Rats Rats, Inbred F344 Rats, Sprague-Dawley Semen Preservation / methods* Sperm Motility / drug effects, physiology Spermatozoa / drug effects*, physiology |
| Grant Support | |
ID/Acronym/Agency:
|
P40 RR016939/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cryoprotective Agents; 107-21-1/Ethylene Glycol; 56-81-5/Glycerol; 57-55-6/Propylene Glycol; 67-68-5/Dimethyl Sulfoxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Differentiation stage-specific analysis of gene function with inducible short hair-pin RNA in differ...
Next Document: Catalytic competence of the Ras-GEF domain of hSos1 requires intra-REM domain interactions mediated ...