Document Detail


Osmotic swelling characteristics of glial cells in the murine hippocampus, cerebellum, and retina in situ.
MedLine Citation:
PMID:  18221375     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glial cells are proposed to play a major role in the ionic and osmotic homeostasis in the CNS. Swelling of glial cells contributes to the development of edema in neural tissue under pathological conditions such as trauma and ischemia. In this study, we compared the osmotic swelling characteristics of murine hippocampal astrocytes, cerebellar Bergmann glial cells, and retinal Müller glial cells in acutely isolated tissue slices in response to hypoosmotic stress and pharmacological blockade of Kir channels. Hypoosmotic challenge induced an immediate swelling of somata in the majority of Bergmann glial cells and hippocampal astrocytes investigated, whereas Müller cell bodies displayed a substantial delay in the onset of swelling and hippocampal astroglial processes remained unaffected. Blockade of Kir channels under isoosmotic conditions had no swelling-inducing effect in Müller cell somata but caused a swelling in brain astrocytic somata and processes. Blockade of Kir channels under hypoosmotic conditions induced an immediate and strong swelling in Müller cell somata, but had no cumulative effect to brain astroglial somata. No regulatory volume decrease could be observed in all cell types. The data suggest that Kir channels are differently implicated in cell volume homeostasis of retinal Müller cells and brain astrocytes and that Müller cells and brain astrocytes differ in their osmotic swelling properties.
Authors:
Petra G Hirrlinger; Antje Wurm; Johannes Hirrlinger; Andreas Bringmann; Andreas Reichenbach
Related Documents :
10088675 - Cells expressing the ng2 antigen contact nodes of ranvier in adult cns white matter.
2865965 - Cloned neoplastic cells from a chemically-induced rat glioma: immunocytochemical charac...
22910795 - Impact of lead and mercuric ions on the interleukin-2-dependent proliferation and survi...
8889215 - Constitutive expression of glutamic acid decarboxylase (gad) by striatal cell lines imm...
16169115 - Microtubules are more stable and more highly acetylated in ethanol-treated hepatic cells.
2933415 - Surface functions during mitosis in rat basophilic leukemia cells.
Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-21
Journal Detail:
Title:  Journal of neurochemistry     Volume:  105     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-01     Completed Date:  2008-06-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  1405-17     Citation Subset:  IM    
Affiliation:
Paul Flechsig Institute of Brain Research, University of Leipzig, Faculty of Medicine, Leipzig, Germany. petra.hirrlinger@medizin.uni-leipzig.de
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Size*
Cerebellum / cytology,  metabolism*
Hippocampus / cytology,  metabolism*
Mice
Mice, Inbred C57BL
Mice, Transgenic
Neuroglia / cytology,  metabolism*
Organ Culture Techniques
Osmosis / physiology
Retina / cytology,  metabolism*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Structure-activity analysis of ginkgolide binding in the glycine receptor pore.
Next Document:  Association of cathepsin E deficiency with the increased territorial aggressive response of mice.