Document Detail

Osmotic swelling characteristics of glial cells in the murine hippocampus, cerebellum, and retina in situ.
MedLine Citation:
PMID:  18221375     Owner:  NLM     Status:  MEDLINE    
Glial cells are proposed to play a major role in the ionic and osmotic homeostasis in the CNS. Swelling of glial cells contributes to the development of edema in neural tissue under pathological conditions such as trauma and ischemia. In this study, we compared the osmotic swelling characteristics of murine hippocampal astrocytes, cerebellar Bergmann glial cells, and retinal Müller glial cells in acutely isolated tissue slices in response to hypoosmotic stress and pharmacological blockade of Kir channels. Hypoosmotic challenge induced an immediate swelling of somata in the majority of Bergmann glial cells and hippocampal astrocytes investigated, whereas Müller cell bodies displayed a substantial delay in the onset of swelling and hippocampal astroglial processes remained unaffected. Blockade of Kir channels under isoosmotic conditions had no swelling-inducing effect in Müller cell somata but caused a swelling in brain astrocytic somata and processes. Blockade of Kir channels under hypoosmotic conditions induced an immediate and strong swelling in Müller cell somata, but had no cumulative effect to brain astroglial somata. No regulatory volume decrease could be observed in all cell types. The data suggest that Kir channels are differently implicated in cell volume homeostasis of retinal Müller cells and brain astrocytes and that Müller cells and brain astrocytes differ in their osmotic swelling properties.
Petra G Hirrlinger; Antje Wurm; Johannes Hirrlinger; Andreas Bringmann; Andreas Reichenbach
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-21
Journal Detail:
Title:  Journal of neurochemistry     Volume:  105     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-01     Completed Date:  2008-06-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  1405-17     Citation Subset:  IM    
Paul Flechsig Institute of Brain Research, University of Leipzig, Faculty of Medicine, Leipzig, Germany.
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MeSH Terms
Cell Size*
Cerebellum / cytology,  metabolism*
Hippocampus / cytology,  metabolism*
Mice, Inbred C57BL
Mice, Transgenic
Neuroglia / cytology,  metabolism*
Organ Culture Techniques
Osmosis / physiology
Retina / cytology,  metabolism*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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