Document Detail

Osmotic stress causes a G1 cell cycle delay and downregulation of Cln3/Cdc28 activity in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  11251821     Owner:  NLM     Status:  MEDLINE    
Moderate hyperosmotic stress on Saccharomyces cerevisiae cells produces a temporary delay at the G1 stage of the cell cycle. This is accompanied by transitory downregulation of CLN1, CLN2 and CLB5 transcript levels, although not of CLN3, which codes for the most upstream activator of the G1/S transition. Osmotic shock to cells synchronized in early G1, when Cln3 is the only cyclin present, causes a delay in cell cycle resumption. This points to Cln3 as being a key cell cycle target for osmotic stress. We have observed that osmotic shock causes downregulation of the kinase activity of Cln3-Cdc28 complexes. This is concomitant with a temporary accumulation of Cln3 protein as a result of increased stability. The effects of the osmotic stress in G1 are not suppressed in CLN3-1 cells with increased kinase activity, as the Cln3-Cdc28 activity in this mutant is still affected by the shock. Although Hog1 is not required for the observed cell cycle arrest in hyperosmotic conditions, it is necessary to resume the cell cycle at KCl concentrations higher than 0.4 M.
G Bellí; E Garí; M Aldea; E Herrero
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  39     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-03-19     Completed Date:  2001-06-14     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1022-35     Citation Subset:  IM    
Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina, Universitat de Lleida, Rovira Roure 44, 25198 Lleida, Spain.
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MeSH Terms
CDC28 Protein Kinase, S cerevisiae / metabolism*
Cyclins / genetics,  metabolism*
Fungal Proteins / genetics,  metabolism*
G1 Phase
Genes, Fungal
Membrane Glycoproteins*
Membrane Proteins / metabolism
Mitogen-Activated Protein Kinases / metabolism
Molecular Chaperones*
Osmotic Pressure
Saccharomyces cerevisiae / cytology,  growth & development,  metabolism*
Saccharomyces cerevisiae Proteins*
Transcription Factors / metabolism
Reg. No./Substance:
0/CLN1 protein, S cerevisiae; 0/CLN2 protein, S cerevisiae; 0/CLN3 protein, S cerevisiae; 0/CLN3 protein, human; 0/Cyclins; 0/Fungal Proteins; 0/Membrane Glycoproteins; 0/Membrane Proteins; 0/Molecular Chaperones; 0/SBF protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors; EC 2.7.10.-/HOG1 protein, S cerevisiae; EC Protein Kinase, S cerevisiae; EC Protein Kinases

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