Document Detail


Osmotic response of mammalian cells: effects of permeating cryoprotectants on nonsolvent volume.
MedLine Citation:
PMID:  8847365     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nonsolvent volume, b, of a cell permits calculation of cell water volume from measurements of total cell volume, and, consequently, it is used extensively in the determination of membrane permeability coefficients for water and solutes and also in simulations of water and solute fluxes during freezing of cells. The nonsolvent volume is most commonly determined from the ordinate intercept of plots of cell volume as a function of the reciprocal of extracellular nonpermeating solute concentration (so-called Boyle-van't Hoff plots). Once derived, b is often assumed to be constant even under conditions that may differ markedly from those under which it was determined. Our aim was to investigate whether this assumption was valid when cells were exposed to the cryoprotectants glycerol, dimethyl sulphoxide (Me2SO), or propane-1,2-diol. Rabbit corneal keratocytes, a fibroblastic cell type, were exposed to 10% (v/v) cryoprotectant for 30 min at 22 degrees C in solutions containing a range of nonpermeating solute concentrations. Cell volumes were determined by an electronic particle sizer and mode volume plotted as an inverse function of the concentration of nonpermeating solute. The cells behaved as osmometers under all conditions studied, but we found no evidence to suggest that the nonsolvent volume of cells was altered by Me2SO or propane-1,2-diol. Glycerol, however, reduced the slope of the Boyle-van't Hoff plot, but this could be ascribed to the failure of the cells to equilibrate fully with the glycerol over the 30 min exposure time; thus, b was unaffected by glycerol. It may be assumed, therefore, that the nonsolvent volume was not influenced by the presence inside cells of any of these nonelectrolyte cryoprotectants.
Authors:
W J Armitage; B K Juss
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  168     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-10-23     Completed Date:  1996-10-23     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  532-38     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, University of Bristol, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Membrane Permeability
Cell Size*
Cornea / cytology*
Cryoprotective Agents / chemistry*
Dimethyl Sulfoxide / chemistry
Glycerol / chemistry
Propylene Glycols / chemistry
Rabbits
Solvents
Time Factors
Water-Electrolyte Balance*
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 0/Propylene Glycols; 0/Solvents; 56-81-5/Glycerol; 67-68-5/Dimethyl Sulfoxide

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