Document Detail


Osmotic pressure measurements of ovalbumin and lysozyme mixtures.
MedLine Citation:
PMID:  3427206     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ovalbumin and lysozyme have been reported to undergo a mixed association in solutions of low ionic strength. Osmotic pressure experiments were performed on ovalbumin and on lysozyme solutions in 0.06 M cacodylate buffer (I = 0.02, pH = 5.8) at 30 and at 37 degrees C. The individual proteins did not undergo any self-associations at either temperature; these measurements indicated that each of the solutions was nonideal. Osmotic pressure experiments on three blends of lysozyme and ovalbumin at 30 and 37 degrees C could be interpreted in two ways. One interpretation was that a nonideal, nonassociating mixture of A and B was present; for the three solutions the mixed nonideal term BAB was negative. A negative nonideal term is usually interpreted as indicating an association. The other interpretation of the data was as a quasi-ideal mixed association of the type A + B in equilibrium AB.
Authors:
B W McCarty; E T Adams
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biophysical chemistry     Volume:  28     ISSN:  0301-4622     ISO Abbreviation:  Biophys. Chem.     Publication Date:  1987 Nov 
Date Detail:
Created Date:  1988-03-02     Completed Date:  1988-03-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  149-59     Citation Subset:  IM    
Affiliation:
Chemistry Department, Texas A&M University, College Station 77843.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Gas
Membranes, Artificial
Muramidase / analysis*
Osmolar Concentration
Ovalbumin / analysis*
Spectrophotometry, Ultraviolet
Ultracentrifugation
Chemical
Reg. No./Substance:
0/Membranes, Artificial; 9006-59-1/Ovalbumin; EC 3.2.1.17/Muramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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