Document Detail

Osmotic loading of in situ chondrocytes in their native environment.
MedLine Citation:
PMID:  21141677     Owner:  NLM     Status:  In-Process    
Changes in the osmotic environment cause changes in volume of isolated cells and cells in tissue explants, and the osmotic environment becomes hypotonic in cartilage diseases such as osteoarthritis (OA). However, it is not known how cells respond to a hypotonic osmotic challenge when situated in the fully intact articular cartilage. A confocal laser scanning microscope was used to image chondrocytes of intact rabbit patellae in an isotonic (300 mOsm) and hypotonic (172 mOsm) immersion medium. Cell volumes were calculated before and 5, 15, 60, 120 and 240 minutes after the change in saline concentration. Local tissue strains and swelling of the entire tissue were estimated from the relative movements of cells and displacements of single cells, respectively. Cell volumes increased rapidly (< or = 5 minutes, p<0.05) by approximately 22%, after which they remained constant for an hour (p>0.05). However, two and four hours post the hypotonic challenge, cell volumes were statistically greater (p<0.05) than those at all earlier time points, and swelling of the entire tissue continued throughout the four hour loading period. The results of our study suggest that osmotic loading induced volume changes of in situ chondrocytes in their native environment occur quickly and continue for hours. Understanding the behaviour of cells in their native environment provides novel insigth into the cell mechanics in ostearthritic joints and so may help understand the onset and progression of this disease.
Rami K Korhonen; Sang-Kuy Han; Walter Herzog
Related Documents :
15082077 - Sertoli cell junctional proteins as early targets for different classes of reproductive...
1327297 - Transcription of the gene for the gap junctional protein connexin43 and expression of f...
9581867 - Estrogen modulation of osteoblastic cell-to-cell communication.
15784337 - Effect of self-microemulsifying drug delivery systems containing labrasol on tight junc...
12930727 - Cholesterol side-chain cleavage cytochrome p450 and 3beta-hydroxysteroid dehydrogenase ...
24158907 - Erf115 controls root quiescent center cell division and stem cell replenishment.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular & cellular biomechanics : MCB     Volume:  7     ISSN:  1556-5297     ISO Abbreviation:  Mol Cell Biomech     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101253756     Medline TA:  Mol Cell Biomech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  125-34     Citation Subset:  IM    
Human Performance Laboratory, Faculty of Kinesiology, University of Calgary, 2500 University Dr NW, Calgary, Alberta, T2N 1N4 Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Grant Support
//Canadian Institutes of Health Research

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cancer cell(s) cycle sequencing reveals universal mechanisms of apoptosis.
Next Document:  On p21 tracking property in cancer cell unravelled bio-digitally in silico. Are apoptosis principles...