Document Detail


Osmotic injury of PC-3 cells by hypertonic NaCl solutions at temperatures above 0 degrees C.
MedLine Citation:
PMID:  15710370     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell injury due to osmotic dehydration, which is regarded as a major cause of injury during freeze-thaw processes, was examined closely using a perfusion microscope. Human prostatic adenocarcinoma cells (PC-3), which were put in a chamber, were subjected to hyperosmotic stresses by perfusing NaCl solutions of varying concentrations into the chamber. Cells were exposed to 2.5 and 4.5M NaCl solutions for 1-60 min by changing the concentrations at 0.2, 1, and 10 M/min. Decrease in cell viability was biphasic: the viability decreased first after the increase in NaCl concentration due to dehydration and then after return to isotonic conditions due to rehydration. Rehydration was substantially more responsible for cell injury than dehydration, which was marked at lower NaCl concentrations and lower temperatures. Injury resulting from contraction was negligible at the 2.5 M NaCl solution. While the hypertonic cell survival, which was determined without a return to isotonic conditions, was almost independent of time of exposure to hyperosmotic concentrations, the post-hypertonic survival after returning to isotonic conditions decreased with increasing exposure time, suggesting that the rehydration-induced injury was a consequence of time-dependent alteration of the plasma membrane. The post-hypertonic survival was lower for higher NaCl concentrations and higher temperatures, which was qualitatively consistent with previous studies. Effects of the rate of concentration change on the post-hypertonic cell survival were observed at 4.5 M; the highest rate of survival was obtained by slower increase and faster decrease in the NaCl concentration. However, the effect was negligible at 2.5 M.
Authors:
Sylwia Zawlodzka; Hiroshi Takamatsu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-12-15
Journal Detail:
Title:  Cryobiology     Volume:  50     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-15     Completed Date:  2005-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  58-70     Citation Subset:  IM    
Affiliation:
Interdisciplinary Graduate School of Engineering Sciences, Kyushu University 6-1 Kasugakoen, Kasuga 816-8580, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Cell Membrane / metabolism
Cell Survival
Cryopreservation
Cryoprotective Agents / pharmacology
Dehydration
Dose-Response Relationship, Drug
Freezing
Humans
Hypertonic Solutions / pharmacology*
Microscopy
Osmolar Concentration
Osmosis
Osmotic Pressure
Perfusion
Saline Solution, Hypertonic / pharmacology*
Sodium Chloride / pharmacology*
Temperature
Time Factors
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 0/Hypertonic Solutions; 0/Saline Solution, Hypertonic; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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