Document Detail


Osmotic gradients induce bio-reminiscent morphological transformations in giant unilamellar vesicles.
MedLine Citation:
PMID:  22586404     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
We report observations of large-scale, in-plane and out-of-plane membrane deformations in giant uni- and multilamellar vesicles composed of binary and ternary lipid mixtures in the presence of net transvesicular osmotic gradients. The lipid mixtures we examined consisted of binary mixtures of DOPC and DPPC lipids and ternary mixtures comprising POPC, sphingomyelin and cholesterol over a range of compositions - both of which produce co-existing phases for selected ranges of compositions at room temperature under thermodynamic equilibrium. In the presence of net osmotic gradients, we find that the in-plane phase separation potential of these mixtures is non-trivially altered and a variety of out-of-plane morphological remodeling events occur. The repertoire of membrane deformations we observe display striking resemblance to their biological counterparts in live cells encompassing vesiculation, membrane fission and fusion, tubulation and pearling, as well as expulsion of entrapped vesicles from multicompartmental giant unilamellar vesicles through large, self-healing transient pores. These observations suggest that the forces introduced by simple osmotic gradients across membrane boundaries could act as a trigger for shape-dependent membrane and vesicle trafficking activities. We speculate that such coupling of osmotic gradients with membrane properties might have provided lipid-mediated mechanisms to compensate for osmotic stress during the early evolution of membrane compartmentalization in the absence of osmoregulatory protein machinery.
Authors:
Kamila Oglęcka; Jeremy Sanborn; Atul N Parikh; Rachel S Kraut
Related Documents :
22836164 - Normal and premature rupture of fetal membranes at term delivery differ in regional che...
20930394 - Effects of pathological conditions on ocular pharmacokinetics of antimicrobial drugs.
22421334 - Compliance profile of the human cornea as measured by atomic force microscopy.
22536074 - Hemocompatibility of stent materials: alterations in electrical parameters of erythrocy...
10662624 - Membrane interaction of influenza virus m1 protein.
23062964 - Influence of azo dye-tio(2) interactions on the filtration performance in a hybrid phot...
Publication Detail:
Type:  Journal Article     Date:  2012-05-04
Journal Detail:
Title:  Frontiers in physiology     Volume:  3     ISSN:  1664-042X     ISO Abbreviation:  Front Physiol     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-15     Completed Date:  2012-10-02     Revised Date:  2013-08-13    
Medline Journal Info:
Nlm Unique ID:  101549006     Medline TA:  Front Physiol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  120     Citation Subset:  -    
Affiliation:
Division of Molecular Genetics and Cell Biology, School of Biological Sciences, Nanyang Technological University Singapore.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Impact of KChIP2 on Cardiac Electrophysiology and the Progression of Heart Failure.
Next Document:  Defects in cytoskeletal signaling pathways, arrhythmia, and sudden cardiac death.