Document Detail


Osmotic and cytotoxic study of vitrification of immature bovine oocytes.
MedLine Citation:
PMID:  8107016     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Four experiments were conducted to determine the composition of a solution suitable for vitrification of immature bovine oocytes. The osmotic and cytotoxic effect of different concentrations (0, 0.25, 0.5 and 1 mol l-1) of nonpermeating solutes (sucrose versus trehalose) were examined. In addition, the effect of permeating cryoprotectants such as glycerol, dimethyl sulfoxide, and propylene glycol (40% w/v) on the viability of oocytes was studied to determine the optimal time of exposure and the most suitable cryoprotectant. Exposure of bovine oocytes to trehalose was less harmful than exposure to sucrose (P < 0.01), and high normospermic fertilization (70%) was achieved after exposure to 0.25 mol trehalose l-1. Propylene glycol was chosen as the cryoprotectant for the vitrification of immature bovine oocytes because of its fast permeating rate and its low cytotoxic effect. The composition of this solution (40% propylene glycol and 0.25 mol trehalose l-1 in PBS containing 4% w/v BSA) appeared to be suitable for vitrification, as the fertilization rate of the vitrified oocytes was 37% (36 of 97).
Authors:
A Arav; D Shehu; M Mattioli
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  99     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1994-03-24     Completed Date:  1994-03-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  353-8     Citation Subset:  IM    
Affiliation:
Istituto di Fisiologia Veterinaria, Universitá di Bologna, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell Survival / drug effects
Cryopreservation / methods*
Cryoprotective Agents*
Female
Fertilization in Vitro
Oocytes / metabolism*
Osmosis
Propylene Glycol
Propylene Glycols
Sucrose
Trehalose
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 0/Propylene Glycols; 57-50-1/Sucrose; 57-55-6/Propylene Glycol; 99-20-7/Trehalose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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