Document Detail


Osmotic adjustment and requirement for sodium in marine protist thraustochytrid.
MedLine Citation:
PMID:  20849566     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A non-invasive ion-selective microelectrode technique was used to elucidate the ionic mechanisms of osmotic adjustment in a marine protist thraustochytrid. Hypoosmotic stress caused significant efflux of Na(+), Cl(-) and K(+) from thraustochytrid cells. Model calculations showed that almost complete osmotic adjustment was achieved within the first 30 min after stress onset. Of these, sodium was the major contributor (more than half of the total osmotic adjustment), with chloride being the second major contributor. The role of K(+) in the process of osmotic adjustment was relatively small. Changes in Ca(2+) and H(+) flux were attributed to intracellular signalling. Ion flux data were confirmed by growth experiments. Thraustochytrium cells showed normal growth patterns even when grown in a sodium-free solution provided the medium osmolality was adjusted by mannitol to one of the seawater. That suggests that the requirement of sodium for thraustochytrid growth cycle is due to its role in cell osmotic adjustment rather than because of the direct Na(+) involvement in cell metabolism. Altogether, these data demonstrate the evidence for turgor regulation in thraustochytrids and suggest that these cells may be grown in the absence of sodium providing that cell turgor is adjusted by some other means.
Authors:
Lana Shabala; Tom McMeekin; Sergey Shabala
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental microbiology     Volume:  11     ISSN:  1462-2920     ISO Abbreviation:  Environ. Microbiol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2010-09-20     Completed Date:  2011-01-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883692     Medline TA:  Environ Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1835-43     Citation Subset:  IM    
Affiliation:
School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, Tas. 7001, Australia.
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MeSH Terms
Descriptor/Qualifier:
Calcium / metabolism
Chlorine / metabolism
Hydrogen / metabolism
Osmotic Pressure
Potassium / metabolism
Sodium / metabolism*
Stramenopiles / growth & development,  metabolism,  physiology*
Water Microbiology*
Chemical
Reg. No./Substance:
1333-74-0/Hydrogen; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-70-2/Calcium; 7782-50-5/Chlorine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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