Document Detail

Osmotic shrinkage as a factor in freezing injury in plant tissue cultures.
MedLine Citation:
PMID:  16659469     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Haplopappus gracilis and Acer saccharum tissue culture cells are extremely sensitive to freezing injury, and exhibit a decrease in survival from 98% at -1 C to 4% at -3 C (Haplopappus) and 92% at -3 C to 13% at -5 C (Acer) when suspended in distilled H(2)O, seeded at -1 C, and then cooled by 0.1 C/minute. Similar results are obtained when cells are suspended in growth medium. The extent of shrinkage of cells during freezing can be duplicated by exposure of the cells to plasmolyzing solutions of nonpenetrating substances (Delta T(f) = 1.86 phivm). Solutions of sucrose and glycerol that produce extensive plasmolysis cause a decrease in survival within 3 to 5 minutes at room temperature, and the higher the molality to which the cell is exposed the greater the injury. Also, the rate of rehydration of the plasmolyzed cell and of the frozen cell affects its survival, with the slower rate being more beneficial. The close correlation between the decrease in survival at subzero temperatures and the decrease in survival when cells are placed in solutions having osmolalities, which could produce the same extent of shrinkage as these killing temperatures, suggests that this shrinkage is related to freezing injury in tissue culture cells.
L E Towill; P Mazur
Related Documents :
758949 - The effects of suspending medium viscosity on erythrocyte deformation and haemolysis in...
17995889 - Survival in food systems of lactobacillus rhamnosus r011 microentrapped in whey protein...
429549 - Production of erythrocytes that contain fetal hemoglobin in anemia. transient in vivo c...
1340509 - Effects of cryopreservation on immune responses: vi. an inexpensive method for freezing...
12125079 - Comparison of fresh and cryopreserved porcine ventral mesencephalon cells transplanted ...
24585969 - A role for reactive oxygen species in the resolution of persistent genomic instability ...
10651729 - Role of p-glycoprotein in all-trans retinoic acid (atra) resistance in acute promyelocy...
20040369 - Involvement of caspase and mapk activities in norcantharidin-induced colorectal cancer ...
2842279 - Localization of four phosphatases in rat liver sinusoidal cells. an enzyme cytochemical...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  57     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1976 Feb 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  290-6     Citation Subset:  -    
The University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences and Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Stem Sensitivity and Ethylene Involvement in Phototropism of Mung Bean.
Next Document:  Peridinin-Chlorophyll a Proteins of the Dinoflagellate Amphidinium carterae (Plymouth 450).