Document Detail


Osmotic Shock Augments Ethanol Stress in Saccharomyces cerevisiae MTCC 2918.
MedLine Citation:
PMID:  22038037     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Yeast cells sense and respond to hypertonicity. Saccharomyces cerevisiae MTCC 2918 was tested for its metabolic status in 1 M NaCl by cell viability analysis, intracellular glycerol content and total antioxidant capacity. Yeast cell viability was maximum in 1 M NaCl and 24 h addition of 1 M NaCl was effective in induction of hyperosmolarity. Increased glycerol contents in cells treated with salt indicated adaptation to osmotic stress with a maximum of 240.87 ± 0.38 mg/g dry weight (DW) at 72 h. The total antioxidant status with 1 M NaCl was 9.29 ± 0.39 mM/g DW at 96 h reflecting free radical quenching to overcome stress with increasing growth period. Considering that pre-adaptation to one type of stress evoked a protective response to other stress factors, we have attempted the cross adaptation of osmotic shock to high ethanol concentrations. In effect, we observed that osmotic shock lowered the cell survival by augmentation of cell toxicity by ethanol due to stress induction during exponential phase. Glycerol accumulation to an order of 470.27 ± 0.53 mg/g DW at 48 h in 1 M NaCl and 12% ethanol indicated that both stresses culminated in membrane disruption further leading to cell burst and contributed to the stress overload.
Authors:
Geraldine S M John; Murugesan Gayathiri; Chellan Rose; Asit B Mandal
Related Documents :
19360307 - The effects of pc-spes on chemosensitive and chemoresistant head and neck cancer cells ...
6323197 - Retention of mitochondrial dna species in somatic cell hybrids using antibiotic selection.
3455197 - Intracellular distribution of a platinum-rhodamine 123 complex in cis-platinum sensitiv...
20347887 - Akt loss in human epithelial cells treated with severe hypoxia.
22215857 - Downregulation of tmem16a calcium-activated chloride channel contributes to cerebrovasc...
9543157 - Stereological evaluation of human spermatogenesis after suppression by testosterone tre...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-30
Journal Detail:
Title:  Current microbiology     Volume:  -     ISSN:  1432-0991     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Biotechnology, CSIR-Central Leather Research Institute (CLRI), Adyar, Chennai, 600020, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Host Identity Impacts Rhizosphere Fungal Communities Associated with Three Alpine Plant Species.
Next Document:  Construction of Gene Library of 20 kb DNAs from Parasporal Crystal in Bacillus thuringiensis Strain...