Document Detail

Osmoregulation of taurine efflux from cultured human breast cancer cells: comparison with volume activated Cl- efflux and regulation by extracellular ATP.
MedLine Citation:
PMID:  16914896     Owner:  NLM     Status:  MEDLINE    
The properties and regulation of volume-activated taurine efflux from MDA-MB-231 and MCF-7 cells have been investigated. Volume-activated taurine release from both cell lines was almost completely inhibited by diidosalicylate. DIDS , was more effective at inhibiting swelling-induced taurine release from MCF-7 than from MDA-MB-231 cells. On the basis of comparing taurine, Cl(-) and I(-) efflux time courses, it appears that volume-activated taurine efflux does not utilize volume-sensitive anion channels in MDA-MB- 231 and MCF-7 cells. Extracellular ATP stimulated volume-activated taurine release from MDA-MB-231 cells but not from MCF-7 cells. The effect of ATP was mimicked by UTP and was dependent upon external calcium and inhibited by suramin. However, suramin inhibited volume-activated taurine efflux from both MDA-MB-231 and MCF-7 cells even in the absence of exogenously added ATP suggesting that it acts directly on the taurine efflux pathway and/or is inhibiting the effect of ATP released from the cells. Volume-activated taurine efflux from MDA-MB-231 cells was stimulated by ionomycin. In contrast, ionomycin had no effect on taurine release from MCF-7 cells. Adenosine also stimulated volume-activated taurine efflux from MDA-MB-231 cells. The results suggest that purines regulate taurine transport in MDA-MB- 231 cells via more than one type of receptor.
David B Shennan; Jean Thomson; Iain F Gow
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-15
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  18     ISSN:  1015-8987     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2006  
Date Detail:
Created Date:  2006-08-17     Completed Date:  2006-09-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  113-22     Citation Subset:  IM    
Department of Bioscience, Royal College, University of Strathclyde, Glasgow, Scotland, UK.
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MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
Adenosine / pharmacology
Adenosine Triphosphate / metabolism,  pharmacology*
Biological Transport / drug effects
Breast Neoplasms / metabolism,  pathology
Cell Line, Tumor
Chloride Channels / drug effects,  metabolism
Chlorides / metabolism*
Dose-Response Relationship, Drug
Iodides / metabolism
Ionomycin / pharmacology
Osmolar Concentration
Suramin / pharmacology
Taurine / metabolism*
Time Factors
Reg. No./Substance:
0/Chloride Channels; 0/Chlorides; 0/Iodides; 107-35-7/Taurine; 145-63-1/Suramin; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 56-65-5/Adenosine Triphosphate; 56092-81-0/Ionomycin; 58-61-7/Adenosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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