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Osmoregulated periplasmic glucans of Erwinia chrysanthemi.
MedLine Citation:
PMID:  11325941     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the initial characterization of the osmoregulated periplasmic glucans (OPGs) of Erwinia chrysanthemi. OPGs are intrinsic components of the bacterial envelope necessary to the pathogenicity of this phytopathogenic enterobacterium (F. Page, S. Altabe, N. Hugouvieux-Cotte-Pattat, J.-M. Lacroix, J. Robert-Baudouy and J.-P. Bohin, J. Bacteriol. 183:0000-0000, 2001 [companion in this issue]). OPGs were isolated by trichloracetic acid treatment and gel permeation chromatography. The synthesis of these compounds appeared to be osmoregulated, since lower amounts of OPGs were produced when bacteria were grown in media of higher osmolarities. However, a large fraction of these OPGs were recovered in the culture medium. Then, these compounds were characterized by compositional analysis, high-performance anion-exchange chromatography, matrix-assisted laser desorption mass spectrometry, and (1)H and (13)C nuclear magnetic resonance analyses. OPGs produced by E. chrysanthemi are very heterogeneous at the level of both backbone structure and substitution of these structures. The degree of polymerization of the glucose units ranges from 5 to 12. The structures are branched, with a linear backbone consisting of beta-1,2-linked glucose units to which a variable number of branches, composed of one glucose residue, are attached by beta-1,6 linkages in a random way. This glucan backbone may be substituted by O-acetyl and O-succinyl ester-linked residues.
Authors:
V Cogez; P Talaga; J Lemoine; J P Bohin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bacteriology     Volume:  183     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-04-30     Completed Date:  2001-05-31     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3127-33     Citation Subset:  IM    
Affiliation:
Unité de Glycobiologie Structurale et Fonctionnelle, UMR USTL-CNRS 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Ion Exchange / methods
Glucans / chemistry,  isolation & purification,  metabolism*
Magnetic Resonance Spectroscopy / methods
Methylation
Osmolar Concentration
Pectobacterium chrysanthemi / growth & development,  metabolism*
Periplasm / metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Chemical
Reg. No./Substance:
0/Glucans
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