Document Detail


Oscillatory recruitment of signaling proteins to cell tips promotes coordinated behavior during cell fusion.
MedLine Citation:
PMID:  19884508     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell-cell communication is essential for coordinating physiological responses in multicellular organisms and is required for various developmental processes, including cell migration, differentiation, and fusion. To facilitate communication, functional differences are usually required between interacting cells, which can be established either genetically or developmentally. However, genetically identical cells in the same developmental state are also capable of communicating, but must avoid self-stimulation. We hypothesized that such cells must alternate their physiological state between signal sending and receiving to allow recognition and behavioral changes. To test this hypothesis, we studied cell communication in the filamentous fungus Neurospora crassa, a simple and experimentally amenable model system. In N. crassa, germinating asexual spores (germlings) of identical genotype chemotropically sense others in close proximity, show attraction-mediated directed growth, and ultimately undergo cell fusion. Here, we report that two proteins required for cell fusion, a MAP kinase (MAK-2) and a protein of unknown molecular function (SO), exhibit rapid oscillatory recruitment to the plasma membranes of interacting germlings undergoing chemotropic interactions via directed growth. Using an inhibitable MAK-2 variant, we show that MAK-2 kinase activity is required both for chemotropic interactions and for oscillation of MAK-2 and SO to opposing cell tips. Thus, N. crassa germlings undergoing chemotropic interactions rapidly alternate between two different physiological states, associated with signal delivery and response. Such spatiotemporal coordination of signaling allows genetically identical and developmentally equivalent cells to avoid self-stimulation and to coordinate their behavior to achieve the beneficial physiological outcome of cell fusion.
Authors:
André Fleissner; Abigail C Leeder; M Gabriela Roca; Nick D Read; N Louise Glass
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-11-02
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  106     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-20     Completed Date:  2009-12-15     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  19387-92     Citation Subset:  IM    
Affiliation:
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA.
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MeSH Terms
Descriptor/Qualifier:
Fungal Proteins / metabolism*
Neurospora crassa / metabolism,  physiology*
Protein Kinases / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
BB/E010741/1//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Fungal Proteins; EC 2.7.-/Protein Kinases; EC 2.7.3.-/protein-histidine kinase
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