Document Detail


Oscillatory phosphorylation of yeast Fus3 MAP kinase controls periodic gene expression and morphogenesis.
MedLine Citation:
PMID:  18976914     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Signal-transduction networks can display complex dynamic behavior such as oscillations in the activity of key components [1-6], but it is often unclear whether such dynamic complexity is actually important for the network's regulatory functions [7, 8]. Here, we found that the mitogen-activated protein kinase (MAPK) Fus3, a key regulator of the yeast mating-pheromone response, undergoes sustained oscillations in its phosphorylation and activation state during continuous pheromone exposure. These MAPK activity oscillations led to corresponding oscillations in mating-gene expression. Oscillations in MAPK activity and gene expression required the negative regulator of G protein signaling Sst2 and partially required the MAPK phosphatase Msg5. Peaks in Fus3 activation correlated with periodic rounds of cell morphogenesis, with each peak preceding the formation of an additional mating projection. Preventing projection formation did not eliminate MAPK oscillation, but preventing MAPK oscillation blocked the formation of additional projections. A mathematical model was developed that reproduced several features of the observed oscillatory dynamics. These observations demonstrate a role for MAPK activity oscillation in driving a periodic downstream response and explain how the pheromone signaling pathway, previously thought to desensitize after 1-3 hr, controls morphology changes that continue for a much longer time.
Authors:
Zoe Hilioti; Walid Sabbagh; Saurabh Paliwal; Adriel Bergmann; Marcus D Goncalves; Lee Bardwell; Andre Levchenko
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-10-30
Journal Detail:
Title:  Current biology : CB     Volume:  18     ISSN:  0960-9822     ISO Abbreviation:  Curr. Biol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-12     Completed Date:  2009-02-03     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  9107782     Medline TA:  Curr Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1700-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biological Clocks*
Enzyme Activation
GTPase-Activating Proteins / metabolism
Gene Expression Regulation, Fungal*
Mitogen-Activated Protein Kinases / metabolism*
Morphogenesis*
Phosphorylation
Protein Tyrosine Phosphatases / metabolism
Saccharomyces cerevisiae / enzymology*,  growth & development
Saccharomyces cerevisiae Proteins / metabolism*
Grant Support
ID/Acronym/Agency:
GM60366/GM/NIGMS NIH HHS; GM69013/GM/NIGMS NIH HHS; GM72024/GM/NIGMS NIH HHS; GM75309/GM/NIGMS NIH HHS; GM76516/GM/NIGMS NIH HHS; GM84332/GM/NIGMS NIH HHS; P50 GM076516/GM/NIGMS NIH HHS; P50 GM076516-020003/GM/NIGMS NIH HHS; R01 GM060366/GM/NIGMS NIH HHS; R01 GM060366-09/GM/NIGMS NIH HHS; R01 GM072024/GM/NIGMS NIH HHS; R01 GM072024-04/GM/NIGMS NIH HHS; R33 GM069013-04/GM/NIGMS NIH HHS; RR020839/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/GTPase-Activating Proteins; 0/SST2 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; EC 2.7.11.24/FUS3 protein, S cerevisiae; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 3.1.3.48/MSG5 protein, S cerevisiae; EC 3.1.3.48/Protein Tyrosine Phosphatases
Comments/Corrections
Erratum In:
Curr Biol. 2008 Dec 9;18(23):1897

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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