| Oscillatory chloride efflux at the pollen tube apex has a role in growth and cell volume regulation and is targeted by inositol 3,4,5,6-tetrakisphosphate. | |
MedLine Citation:
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PMID: 12215517 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oscillatory growth of pollen tubes has been correlated with oscillatory influxes of the cations Ca(2+), H(+), and K(+). Using an ion-specific vibrating probe, a new circuit was identified that involves oscillatory efflux of the anion Cl(-) at the apex and steady influx along the tube starting at 12 microm distal to the tip. This spatial coupling of influx and efflux sites predicts that a vectorial flux of Cl(-) ion traverses the apical region. The Cl(-) channel blockers 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) and 5-nitro-2-(3-phenylpropylamino)benzoic acid completely inhibited tobacco pollen tube growth at 80 and 20 microM, respectively. Cl(-) channel blockers also induced increases in apical cell volume. The apical 50 micro m of untreated pollen tubes had a mean cell volume of 3905 +/- 75 microm(3). DIDS at 80 microM caused a rapid and lethal cell volume increase to 6206 +/- 171 microm(3), which is at the point of cell bursting at the apex. DIDS was further demonstrated to disrupt Cl(-) efflux from the apex, indicating that Cl(-) flux correlates with pollen tube growth and cell volume status. The signal encoded by inositol 3,4,5,6-tetrakisphosphate [Ins(3,4,5,6)P(4)] antagonized pollen tube growth, induced cell volume increases, and disrupted Cl(-) efflux. Ins(3,4,5,6)P(4) decreased the mean growth rate by 85%, increased the cell volume to 5997 +/- 148 microm(3), and disrupted normal Cl(-) efflux oscillations. These effects were specific for Ins(3,4,5,6)P(4) and were not mimicked by either Ins(1,3,4,5)P(4) or Ins(1,3,4,5,6)P(5). Growth correlation analysis demonstrated that cycles of Cl(-) efflux were coupled to and temporally in phase with cycles of growth. A role for Cl(-) flux in the dynamic cellular events during growth is assessed. Differential interference contrast microscopy and kymographic analysis of individual growth cycles revealed that vesicles can advance transiently to within 2 to 4 microm of the apex during the phase of maximally increasing Cl(-) efflux, which temporally overlaps the phase of cell elongation during the growth cycle. In summary, these investigations indicate that Cl(-) ion dynamics are an important component in the network of events that regulate pollen tube homeostasis and growth. |
Authors:
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Laura Zonia; Sofia Cordeiro; Jaroslav Tupý; José A Feijó |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Plant cell Volume: 14 ISSN: 1040-4651 ISO Abbreviation: Plant Cell Publication Date: 2002 Sep |
Date Detail:
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Created Date: 2002-09-06 Completed Date: 2002-12-27 Revised Date: 2010-09-20 |
Medline Journal Info:
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Nlm Unique ID: 9208688 Medline TA: Plant Cell Country: United States |
Other Details:
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Languages: eng Pagination: 2233-49 Citation Subset: IM |
Affiliation:
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Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Pernikarce 15, 160 00 Prague 6, Czech Republic. zonia@ueb.cas.cz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
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pharmacology Cell Division / drug effects Cell Size / drug effects Chloride Channels / metabolism Chlorides / metabolism* Inositol Phosphates / pharmacology* Lilium / cytology, drug effects, growth & development Niflumic Acid / pharmacology Nitrobenzoates / pharmacology Pollen / drug effects, growth & development*, metabolism Tobacco / cytology, drug effects, growth & development |
| Chemical | |
Reg. No./Substance:
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0/Chloride Channels; 0/Chlorides; 0/Inositol Phosphates; 0/Nitrobenzoates; 0/inositol-1,3,4,5,6-pentakisphosphate; 102850-29-3/inositol-1,3,4,5-tetrakisphosphate; 107254-86-4/5-nitro-2-(3-phenylpropylamino)benzoic acid; 112791-61-4/inositol-3,4,5,6-tetrakisphosphate; 4394-00-7/Niflumic Acid; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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