Document Detail

Oscillation in the activities of MEK/ERK1/2 during cardiopulmonary bypass in pigs.
MedLine Citation:
PMID:  11490347     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Because cardiopulmonary bypass (CPB) is associated with edema and vasoreactive dysfunction and ERK1/2 pathway is involved in vascular contractility and permeability, a time course study was performed to monitor MEK/ERK1/2/Elk-1 activities during CPB. METHODS: Pigs were subjected to normothermic CPB for 90 minutes followed by post-CPB perfusion for 180 minutes. Atrial myocardium was sampled before CPB, 5 minutes after CPB onset, 5 minutes after weaning from CPB, and at the end of post-CPB. Skeletal muscle and mesenteric vessels samples were harvested before CPB, 5 minutes after CPB institution, and every 30 minutes thereafter to the end of post-CPB. Samples were analyzed by immunoblotting and immunofluorescence microscopy with the use of specific antibodies against active (phosphorylated) forms of ERK1/2, MEK1/2, and Elk-1. RESULTS: Pigs that were subjected to CPB showed an increase in phospho-ERK1/2 after 30 minutes of CPB, followed by a decrease after 90 minutes. Another phosphorylation peak was observed 30 to 60 minutes of post-CPB, followed by a decrease to below baseline at the end of reperfusion. MEK1/2 and Elk-1 activation profiles paralleled ERK1/2 activity peaks. Control samples showed no significant increase above basal levels. CONCLUSIONS: Activation of MEK/ERK1/2/Elk-1 pathways closely follows major CPB surgical manipulations (institution and termination) and could be related to morbidity during and after CPB.
E G Araujo; C Bianchi; R Faro; F W Sellke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Surgery     Volume:  130     ISSN:  0039-6060     ISO Abbreviation:  Surgery     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-08-07     Completed Date:  2001-09-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0417347     Medline TA:  Surgery     Country:  United States    
Other Details:
Languages:  eng     Pagination:  182-91     Citation Subset:  AIM; IM    
Division of Cardiothoracic Surgery, Department of Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02115, USA.
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MeSH Terms
Blood Gas Analysis
Blood Pressure
Blotting, Western
Cardiopulmonary Bypass*
DNA-Binding Proteins*
Enzyme Activation / physiology
Fluorescent Antibody Technique
Heart Atria / enzymology
MAP Kinase Signaling System / physiology*
Mesenteric Arteries / enzymology
Mesenteric Veins / enzymology
Mitogen-Activated Protein Kinase 1 / analysis,  metabolism*
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases / analysis,  metabolism
Muscle, Skeletal / enzymology
Myocardium / enzymology
Protein-Serine-Threonine Kinases / analysis,  metabolism*
Proto-Oncogene Proteins / metabolism
Transcription Factors*
ets-Domain Protein Elk-1
Grant Support
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Proto-Oncogene Proteins; 0/Transcription Factors; 0/ets-Domain Protein Elk-1; EC Kinases; EC Protein Kinase 1; EC Protein Kinase 3; EC Protein Kinases

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