| Oscillation in the activities of MEK/ERK1/2 during cardiopulmonary bypass in pigs. | |
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MedLine Citation:
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PMID: 11490347 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Because cardiopulmonary bypass (CPB) is associated with edema and vasoreactive dysfunction and ERK1/2 pathway is involved in vascular contractility and permeability, a time course study was performed to monitor MEK/ERK1/2/Elk-1 activities during CPB. METHODS: Pigs were subjected to normothermic CPB for 90 minutes followed by post-CPB perfusion for 180 minutes. Atrial myocardium was sampled before CPB, 5 minutes after CPB onset, 5 minutes after weaning from CPB, and at the end of post-CPB. Skeletal muscle and mesenteric vessels samples were harvested before CPB, 5 minutes after CPB institution, and every 30 minutes thereafter to the end of post-CPB. Samples were analyzed by immunoblotting and immunofluorescence microscopy with the use of specific antibodies against active (phosphorylated) forms of ERK1/2, MEK1/2, and Elk-1. RESULTS: Pigs that were subjected to CPB showed an increase in phospho-ERK1/2 after 30 minutes of CPB, followed by a decrease after 90 minutes. Another phosphorylation peak was observed 30 to 60 minutes of post-CPB, followed by a decrease to below baseline at the end of reperfusion. MEK1/2 and Elk-1 activation profiles paralleled ERK1/2 activity peaks. Control samples showed no significant increase above basal levels. CONCLUSIONS: Activation of MEK/ERK1/2/Elk-1 pathways closely follows major CPB surgical manipulations (institution and termination) and could be related to morbidity during and after CPB. |
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Authors:
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E G Araujo; C Bianchi; R Faro; F W Sellke |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Surgery Volume: 130 ISSN: 0039-6060 ISO Abbreviation: Surgery Publication Date: 2001 Aug |
Date Detail:
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Created Date: 2001-08-07 Completed Date: 2001-09-06 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0417347 Medline TA: Surgery Country: United States |
Other Details:
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Languages: eng Pagination: 182-91 Citation Subset: AIM; IM |
Affiliation:
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Division of Cardiothoracic Surgery, Department of Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02115, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Gas Analysis Blood Pressure Blotting, Western Cardiopulmonary Bypass* DNA-Binding Proteins* Enzyme Activation / physiology Female Fluorescent Antibody Technique Heart Atria / enzymology MAP Kinase Signaling System / physiology* Male Mesenteric Arteries / enzymology Mesenteric Veins / enzymology Mitogen-Activated Protein Kinase 1 / analysis, metabolism* Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinases / analysis, metabolism Muscle, Skeletal / enzymology Myocardium / enzymology Periodicity Phosphorylation Protein-Serine-Threonine Kinases / analysis, metabolism* Proto-Oncogene Proteins / metabolism Swine Transcription Factors* ets-Domain Protein Elk-1 |
| Grant Support | |
ID/Acronym/Agency:
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R01 HL-46716/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Proto-Oncogene Proteins; 0/Transcription Factors; 0/ets-Domain Protein Elk-1; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3; EC 2.7.11.24/Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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