Document Detail


Orthologues of the anaphase-promoting complex/cyclosome coactivators Cdc20p and Cdh1p are important for mitotic progression and morphogenesis in Candida albicans.
MedLine Citation:
PMID:  21398510     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The conserved anaphase-promoting complex/cyclosome (APC/C) system mediates protein degradation during mitotic progression. Conserved coactivators Cdc20p and Cdh1p regulate the APC/C during early to late mitosis and G(1) phase. Candida albicans is an important fungal pathogen of humans, and it forms highly polarized cells when mitosis is blocked through depletion of the polo-like kinase Cdc5p or other treatments. However, the mechanisms governing mitotic progression and associated polarized growth in the pathogen are poorly understood. In order to gain insights into these processes, we characterized C. albicans orthologues of Cdc20p and Cdh1p. Cdc20p-depleted cells were blocked in early or late mitosis with elevated levels of Cdc5p and the mitotic cyclin Clb2p, suggesting that Cdc20p is essential and has some conserved functions during mitosis. However, the yeast cells formed highly polarized buds in contrast to the large doublets of S. cerevisiae cdc20 mutants, implying a distinct role in morphogenesis. In comparison, cdh1Δ/cdh1Δ cells were viable but showed enrichment of Clb2p and Cdc5p, suggesting that Cdh1p may influence mitotic exit. The cdh1Δ/cdh1Δ phenotype was pleiotropic, consisting of normal or enlarged yeast, pseudohyphae, and some elongated buds, whereas S. cerevisiae cdh1Δ yeast cells were reduced in size. Thus, C. albicans Cdh1p may have some distinct functions. Finally, absence of Cdh1p or Cdc20p had a minor or no effect on hyphal development, respectively. Overall, the results suggest that Cdc20p and Cdh1p may be APC/C activators that are important for mitosis but also morphogenesis in C. albicans. Their novel features imply additional variations in function and underscore rewiring in the emerging mitotic regulatory networks of the pathogen.
Authors:
Hsini Chou; Amandeep Glory; Catherine Bachewich
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-03-11
Journal Detail:
Title:  Eukaryotic cell     Volume:  10     ISSN:  1535-9786     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-03     Completed Date:  2011-10-31     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  696-709     Citation Subset:  IM    
Affiliation:
Department of Biology, Concordia University, 7141 Sherbrooke St. West, Montreal, QC H4B 1R6, Canada.
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MeSH Terms
Descriptor/Qualifier:
Candida albicans / cytology*,  growth & development*,  metabolism
Cell Cycle Proteins / genetics,  metabolism*
Fungal Proteins / genetics,  metabolism*
Mitosis*
Morphogenesis / genetics
Nuclear Proteins / genetics,  metabolism
Ubiquitin-Protein Ligase Complexes / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Fungal Proteins; 0/Nuclear Proteins; EC 6.3.2.19/Ubiquitin-Protein Ligase Complexes; EC 6.3.2.19/anaphase-promoting complex
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