| Origin of the vertebrate visual cycle: III. Distinct distribution of RPE65 and beta-carotene 15,15'-monooxygenase homologues in Ciona intestinalis. | |
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MedLine Citation:
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PMID: 16544957 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We previously identified three genes that encode putative visual cycle proteins that are homologues of retinal G-protein coupled receptor (Ci-opsin3), cellular retinaldehyde-binding protein (Ci-CRALBP) and beta-carotene 15,15'-monooxygenase (Ci-BCO) in the ascidian Ciona intestinalis. Ci-opsin3 and Ci-CRALBP are localized in both ocellus photoreceptor cells and surrounding non-photoreceptor cells in the brain vesicle of the larva. In the present study, we investigated the possible role and evolutionary origin of the BCO/RPE65 family in the visual cycle by analyzing Ci-BCO localization by immunohistochemistry and by identifying a novel gene that encodes a homologue of retinal pigment epithelium-specific 65 kDa protein (Ci-RPE65) in C. intestinalis. In situ hybridization and expressed sequence tag (EST) profiles consistently suggest that Ci-RPE65 is not significantly expressed in the ocellus and brain vesicle of the larva. Ci-RPE65 is expressed in the neural complex, a photoreceptor organ of the adult ascidian, at a level comparable to that of Ci-opsin3 and Ci-CRALBP. Ci-RPE65 is also expressed in various adult tissues, including the gill, body wall and intestine, suggesting that Ci-RPE65 plays a role in addition to that in the visual cycle. In contrast, Ci-BCO is predominantly localized in ocellus photoreceptor cells of the larva. The larval visual cycle seems to use Ci-opsin3 as a photo-isomerase. Our results also suggest that the RPE65-dependent visual cycle is used in the adult photoreceptors of a primitive chordate. |
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Authors:
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Noriko Takimoto; Takehiro Kusakabe; Takeo Horie; Yuki Miyamoto; Motoyuki Tsuda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Photochemistry and photobiology Volume: 82 ISSN: 0031-8655 ISO Abbreviation: Photochem. Photobiol. Publication Date: 2006 Nov-Dec |
Date Detail:
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Created Date: 2007-03-26 Completed Date: 2007-04-30 Revised Date: 2012-05-24 |
Medline Journal Info:
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Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: United States |
Other Details:
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Languages: eng Pagination: 1468-74 Citation Subset: IM |
Affiliation:
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Department of Life Science, Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Ako-gun, Hyogo 678-1297, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Ciona intestinalis / enzymology* Conserved Sequence DNA Primers Expressed Sequence Tags Humans Molecular Sequence Data Phylogeny Sequence Alignment Sequence Homology, Amino Acid Vertebrates / classification, genetics Vision, Ocular beta-Carotene 15,15'-Monooxygenase / chemistry, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; EC 1.14.99.36/beta-Carotene 15,15'-Monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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