Document Detail


Origin and progeny of reactive gliosis: A source of multipotent cells in the injured brain.
MedLine Citation:
PMID:  18299565     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reactive gliosis is the universal reaction to brain injury, but the precise origin and subsequent fate of the glial cells reacting to injury are unknown. Astrocytes react to injury by hypertrophy and up-regulation of the glial-fibrillary acidic protein (GFAP). Whereas mature astrocytes do not normally divide, a subpopulation of the reactive GFAP(+) cells does so, prompting the question of whether the proliferating GFAP(+) cells arise from endogenous glial progenitors or from mature astrocytes that start to proliferate in response to brain injury. Here we show by genetic fate mapping and cell type-specific viral targeting that quiescent astrocytes start to proliferate after stab wound injury and contribute to the reactive gliosis and proliferating GFAP(+) cells. These proliferating astrocytes remain within their lineage in vivo, while a more favorable environment in vitro revealed their multipotency and capacity for self-renewal. Conversely, progenitors present in the adult mouse cerebral cortex labeled by NG2 or the receptor for the platelet-derived growth factor (PDGFRalpha) did not form neurospheres after (or before) brain injury. Taken together, the first fate-mapping analysis of astrocytes in the adult mouse cerebral cortex shows that some astrocytes acquire stem cell properties after injury and hence may provide a promising cell type to initiate repair after brain injury.
Authors:
Annalisa Buffo; Inmaculada Rite; Pratibha Tripathi; Alexandra Lepier; Dilek Colak; Ana-Paula Horn; Tetsuji Mori; Magdalena Götz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-25
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  105     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-03-05     Completed Date:  2008-04-18     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3581-6     Citation Subset:  IM    
Affiliation:
Institute for Stem Cell Research, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstrasse 1, 85764 Neuherberg/Munich, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / cytology,  physiology*
Brain Injuries / pathology*
Cell Lineage
Cells, Cultured
Cerebral Cortex
Glial Fibrillary Acidic Protein
Gliosis / pathology*
Mice
Mice, Inbred Strains
Pluripotent Stem Cells / cytology*
Wounds, Stab
Chemical
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein
Comments/Corrections

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