Document Detail

Origin of pancreatic endocrine cells from biliary duct epithelium.
MedLine Citation:
PMID:  18810318     Owner:  NLM     Status:  MEDLINE    
We describe an explant culture system to study the formation of pancreatic-type endocrine cells by the biliary tract. In this model, beta-cells and other endocrine cells appear in the biliary duct epithelium and their number increases. Evidence for an origin from the duct epithelium is threefold. Firstly, differentiating cells transiently co-express insulin and bind Dolichos lectin. Secondly, beta-cells in cultures isolated from Alb-Cre-R26R-LacZ mice are beta-galactosidase positive. Thirdly, co-culture of biliary epithelium and ROSA26 pancreatic buds shows that endocrine cells do not migrate from the pancreas. The expression of the pancreatic transcription factors Pdx1, HNF6 and Sox9 is widespread, as is Hes1, which represses endocrine development, while that of Ngn3, which is a proendocrine transcription factor, is transient, consistent with an early stage of endocrine cell differentiation. Nicotinamide will increase the number of beta-cells formed, while EGF+LIF completely inhibits their formation.
D Eberhard; D Tosh; J M W Slack
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  65     ISSN:  1420-9071     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-06     Completed Date:  2009-01-19     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  3467-80     Citation Subset:  IM    
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MeSH Terms
Antigens, Differentiation / analysis
Basic Helix-Loop-Helix Transcription Factors / metabolism
Bile Ducts, Extrahepatic / cytology*
Cell Differentiation
Cell Division
Cell Lineage
Coculture Techniques
Endoderm / cytology
Epithelial Cells / cytology*,  drug effects,  metabolism
Hepatocyte Nuclear Factor 6 / metabolism
Homeodomain Proteins / metabolism
Insulin / biosynthesis
Insulin-Secreting Cells / cytology,  drug effects,  metabolism
Intercellular Signaling Peptides and Proteins / pharmacology
Islets of Langerhans / cytology*,  embryology
Mice, Inbred C57BL
Mice, Transgenic
Nerve Tissue Proteins / metabolism
Organ Culture Techniques / methods*
Plant Lectins / metabolism
Receptors, Mitogen / metabolism
Trans-Activators / metabolism
Grant Support
G0300415//Medical Research Council; G0500220//Medical Research Council; //Medical Research Council; //Wellcome Trust
Reg. No./Substance:
0/Antigens, Differentiation; 0/Basic Helix-Loop-Helix Transcription Factors; 0/Dolichos biflorus lectin receptor; 0/Hepatocyte Nuclear Factor 6; 0/Hes1 protein, mouse; 0/Homeodomain Proteins; 0/Insulin; 0/Intercellular Signaling Peptides and Proteins; 0/Nerve Tissue Proteins; 0/Neurog3 protein, mouse; 0/Onecut1 protein, mouse; 0/Plant Lectins; 0/Receptors, Mitogen; 0/Trans-Activators; 0/dolichos biflorus agglutinin; 0/pancreatic and duodenal homeobox 1 protein

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