Document Detail


Origin of outgrowth from isolated glomeruli in culture.
MedLine Citation:
PMID:  7323416     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The primary cellular outgrowth from isolated glomeruli was identified by examinations of the ultrastructural changes occurring in the three glomerular cell types during culture and by light and electron microscopic autoradiographic preparations of isolated glomeruli incubated with 3H-thymidine. It is demonstrated that the visceral epithelial cells constitute the majority of cells in the primary glomerular outgrowth and that another cell type probably originates from the mesangial cells. A method is described to obtain rather pure cultures of proliferating visceral epithelial cells suitable for further studies of their metabolic properties.
Authors:
J O Nørgaard
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Renal physiology     Volume:  3     ISSN:  0378-5858     ISO Abbreviation:  Ren Physiol     Publication Date:  1980  
Date Detail:
Created Date:  1982-03-22     Completed Date:  1982-03-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7901911     Medline TA:  Ren Physiol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  220-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Cell Division
Cell Line
Culture Techniques
Endothelium / physiology
Epithelium / physiology
Kidney Glomerulus / blood supply,  physiology*,  ultrastructure
Microscopy, Electron
Swine
Thymidine / metabolism,  pharmacology
Time Factors
Chemical
Reg. No./Substance:
50-89-5/Thymidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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