| Origin and occurrence of human chorionic gonadotropin beta-subunit core fragment. | |
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MedLine Citation:
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PMID: 2459607 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human chorionic gonadotropin (hCG) beta-subunit core fragment (beta-fragment) is present in the urine of pregnant individuals as well as those with trophoblast disease and certain other cancers at concentrations 0.8 (early pregnancy) to 7 (second trimester pregnancy)-fold greater than that of hCG. The core fragment may be directly secreted by trophoblast tissue into the circulation or possibly originates from peripheral degradation of circulating hormone by the kidney. We examined the former hypothesis. We examined 24-h organ cultures of trophoblast tissue from first, second, and third trimester pregnancy. The media from this tissue contained hCG, free beta-subunit, and beta-fragment. The amount of beta-fragment present exceeded that of hCG, as was observed in second and third trimester pregnancy urine. The beta-fragment immunoreactive material produced by trophoblast tissue was compared to a standard preparation of urinary beta-fragment. The material in medium was identical to the standard beta-fragment in its elution pattern from a gel filtration column, from a reverse-phase HPLC column, from an ion-exchange gel, and from an immobilized lectin affinity column, and also by electrophoresis and immunoblotting with fragment-reactive monoclonal antibodies. We conclude that beta-fragment can also originate directly from trophoblast tissue, and could be the principal hCG beta-immunoreactive molecule secreted. |
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Authors:
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L A Cole; S Birken |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular endocrinology (Baltimore, Md.) Volume: 2 ISSN: 0888-8809 ISO Abbreviation: Mol. Endocrinol. Publication Date: 1988 Sep |
Date Detail:
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Created Date: 1988-11-21 Completed Date: 1988-11-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8801431 Medline TA: Mol Endocrinol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 825-30 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut 06510. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chorionic Gonadotropin
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urine* Chorionic Gonadotropin, beta Subunit, Human Chromatography, High Pressure Liquid Female Humans Immunosorbent Techniques Organ Culture Techniques Peptide Fragments / urine* Pregnancy Trophoblasts / secretion |
| Grant Support | |
ID/Acronym/Agency:
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CA-44131/CA/NCI NIH HHS; HD-15454/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 0/Chorionic Gonadotropin, beta Subunit, Human; 0/Peptide Fragments |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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