Document Detail


Origin of neointimal cells in arteriovenous fistulae: bone marrow, artery, or the vein itself?
MedLine Citation:
PMID:  21517994     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To elucidate the source of neointimal cells, experimental fistulas were created in Lewis wild-type (WT) and transgenic rats that constitutively expressed the green fluorescent protein (GFP) in all tissues. Arteriovenous fistula (AVFs) were created by anastomosing the left renal vein to the abdominal aorta. The contribution of bone marrow (BM)-derived cells to the AVF neointima was examined in lethally irradiated WT rats that had been rescued with GFP BM cells. Neointimal cells in these chimeric rats were mostly GFP negative indicating the non-BM origin of those cells. Then, the contribution of arterial cells to the AVF neointima was assessed in a fistula made with a GFP aorta that had been implanted orthotopically into a WT rat. Most of the neointimal cells were also GFP negative demonstrating that AVF neointimal cells are not derived from the feeding artery. Finally to study local resident cells contribution to the formation of neointimal lesions, a composite fistula was created by interposing a GFP vein between the renal vein and the aorta in a WT recipient rat. GFP neointimal cells were only found in the transplanted vein. This study suggests that neointimal cells originate from the local resident cells in the venous limb of the fistula.
Authors:
Nikolaos Skartsis; Eddie Manning; Yuntao Wei; Omaida C Velazquez; Zhao-Jun Liu; Pascal J Goldschmidt-Clermont; Loay H Salman; Arif Asif; Roberto I Vazquez-Padron
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Seminars in dialysis     Volume:  24     ISSN:  1525-139X     ISO Abbreviation:  Semin Dial     Publication Date:    2011 Mar-Apr
Date Detail:
Created Date:  2011-04-26     Completed Date:  2011-09-01     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  8911629     Medline TA:  Semin Dial     Country:  United States    
Other Details:
Languages:  eng     Pagination:  242-8     Citation Subset:  IM    
Copyright Information:
© 2011 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arteries / cytology*,  pathology
Arteriovenous Shunt, Surgical* / adverse effects
Bone Marrow Cells / pathology*
Green Fluorescent Proteins / metabolism
Microscopy, Confocal
Neointima / pathology*
Rats
Rats, Inbred Lew / genetics
Rats, Transgenic
Stem Cells
Veins / cytology*,  pathology
Grant Support
ID/Acronym/Agency:
1K01HL096413-01/HL/NHLBI NIH HHS; K01 HL096413/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins

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