| Origin of individuality of two daughter cells during the division process examined by the simultaneous measurement of growth and swimming property using an on-chip single-cell cultivation system. | |
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MedLine Citation:
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PMID: 17496044 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We examined the origin of individuality of two daughter cells born from an isolated single Escherichia coli mother cell during its cell division process by monitoring the change in its swimming behavior and tumbling frequency using an on-chip single-cell cultivation system. By keeping the isolated condition of an observed single cell, we compared its growth and swimming property within a generation and over up to seven generations. It revealed that running speed decreased as cell length smoothly increased within each generation, whereas tumbling frequency fluctuated among generations. Also found was an extraordinary tumbling mode characterized by the prolonged duration of pausing in predivisional cells after cell constriction. The observed prolonged pausing may imply the coexistence of two distinct control systems in a predivisional cell, indicating that individuality of daughter cells emerges after a mother cell initiates constriction and before it gets physically separated into two new cell bodies. |
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Authors:
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Senkei Umehara; Ippei Inoue; Yuichi Wakamoto; Kenji Yasuda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-05-11 |
Journal Detail:
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Title: Biophysical journal Volume: 93 ISSN: 0006-3495 ISO Abbreviation: Biophys. J. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-07-09 Completed Date: 2007-10-16 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: 1061-7 Citation Subset: IM |
Affiliation:
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Department of Biomedical Information, Division of Biosystems, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Tokyo 101-0062, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacteria
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cytology Bacterial Physiological Phenomena Cell Culture Techniques / methods Cell Division / physiology* Cell Movement / physiology* Cells / cytology* Genes, Reporter Green Fluorescent Proteins / analysis Models, Biological Software |
| Chemical | |
Reg. No./Substance:
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147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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