Document Detail


Origin of extracellular matrix synthesis during coronary repair.
MedLine Citation:
PMID:  9054763     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Coronary injury triggers differentiation of activated adventitial fibroblasts to myofibroblasts, which may contribute to neointimal formation and vascular remodeling. Accordingly, the purpose of this study was to examine the cellular origin of the enhanced synthesis of extracellular matrix proteins during coronary repair. METHODS AND RESULTS: The time course and localization of collagen and elastin expression were examined by in situ hybridization and immunohistochemistry in porcine coronary arteries after balloon-induced injury. Procollagen-alpha 1(I) transcripts and intracellular type I procollagen protein increased in the adventitia within 2 days after injury. This was followed by a sustained synthesis of type I procollagen in neointima beginning at 7 days and the extracellular accumulation of type I collagen in both layers. The origin of synthetic cells was further examined by colocalization of type I procollagen and bromodeoxyuridine labeling to activated adventitial cells, which translocated to neointima. Neointimal cells exhibited sustained synthetic activity manifested by the presence of type I procollagen and elastin at 3 months after injury. In contrast, the media showed only minor changes in the synthesis of collagen or elastin throughout coronary repair. CONCLUSIONS: Activated adventitial fibroblasts are endowed with synthetic capabilities after coronary injury. They express type I procollagen, with some of them translocating to the intima, where they continue to synthesize procollagen. The accumulation of type I collagen is evident in the adventitia and neointima, whereas elastin accumulates mainly in neointima. These findings support the involvement of adventitial fibroblasts in coronary repair and remodeling after endoluminal injury.
Authors:
Y Shi; J E O'Brien; L Ala-Kokko; W Chung; J D Mannion; A Zalewski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Circulation     Volume:  95     ISSN:  0009-7322     ISO Abbreviation:  Circulation     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-04-03     Completed Date:  1997-04-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0147763     Medline TA:  Circulation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  997-1006     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine (Cardiology), Thomas Jefferson University, Philadelphia, Pa 19107, USA. shi1@jeflin.tju.edu
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MeSH Terms
Descriptor/Qualifier:
Angioplasty, Balloon / adverse effects
Animals
Base Sequence
Collagen / biosynthesis*
Coronary Vessels / injuries*,  metabolism*,  pathology
DNA Primers
Elastin / biosynthesis*
In Situ Hybridization
Polymerase Chain Reaction
Procollagen / analysis,  biosynthesis
RNA, Messenger / biosynthesis
Recurrence
Swine
Transcription, Genetic*
Tropoelastin / biosynthesis
Wound Healing
Grant Support
ID/Acronym/Agency:
HL-44150/HL/NHLBI NIH HHS; N01-HD-6-2915/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Procollagen; 0/RNA, Messenger; 0/Tropoelastin; 9007-34-5/Collagen; 9007-58-3/Elastin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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