| Orientation determination of interfacial beta-sheet structures in situ. | |
| | |
MedLine Citation:
|
PMID: 20504035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Structural information such as orientations of interfacial proteins and peptides is important for understanding properties and functions of such biological molecules, which play crucial roles in biological applications and processes such as antimicrobial selectivity, membrane protein activity, biocompatibility, and biosensing performance. The alpha-helical and beta-sheet structures are the most widely encountered secondary structures in peptides and proteins. In this paper, for the first time, a method to quantify the orientation of the interfacial beta-sheet structure using a combined attenuated total reflectance Fourier transformation infrared spectroscopic (ATR-FTIR) and sum frequency generation (SFG) vibrational spectroscopic study was developed. As an illustration of the methodology, the orientation of tachyplesin I, a 17 amino acid peptide with an antiparallel beta-sheet, adsorbed to polymer surfaces as well as associated with a lipid bilayer was determined using the regular and chiral SFG spectra, together with polarized ATR-FTIR amide I signals. Both the tilt angle (theta) and the twist angle (psi) of the beta-sheet at interfaces are determined. The developed method in this paper can be used to obtain in situ structural information of beta-sheet components in complex molecules. The combination of this method and the existing methodology that is currently used to investigate alpha-helical structures will greatly broaden the application of optical spectroscopy in physical chemistry, biochemistry, biophysics, and structural biology. |
| | |
Authors:
|
Khoi Tan Nguyen; John Thomas King; Zhan Chen |
Related Documents
:
|
17714685 - Permeation of a beta-heptapeptide derivative across phospholipid bilayers. 16173725 - Residue requirements for helical folding in short alpha/beta-peptides: crystallographic... 7925395 - Antibodies for the immunochemistry of the human beta 3-adrenergic receptor. 19229915 - High-resolution structural characterization of a helical alpha/beta-peptide foldamer bo... 2820805 - Modulation of retinal transducin and phosphodiesterase activities by synthetic peptides... 15300485 - Habitat structural complexity mediates the foraging success of multiple predator species. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: The journal of physical chemistry. B Volume: 114 ISSN: 1520-5207 ISO Abbreviation: J Phys Chem B Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-06-24 Completed Date: 2010-09-22 Revised Date: 2011-08-01 |
Medline Journal Info:
|
Nlm Unique ID: 101157530 Medline TA: J Phys Chem B Country: United States |
Other Details:
|
Languages: eng Pagination: 8291-300 Citation Subset: IM |
Affiliation:
|
Department of Chemistry, 930 North University Avenue, University of Michigan, Ann Arbor, Michigan 48109, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Algorithms Amino Acid Sequence Anti-Infective Agents / chemistry* Antimicrobial Cationic Peptides / chemistry* DNA-Binding Proteins / chemistry* Lipid Bilayers / chemistry, metabolism Molecular Sequence Data Peptides, Cyclic / chemistry* Polymers / chemistry Protein Structure, Secondary Spectroscopy, Fourier Transform Infrared Spectrum Analysis, Raman |
| Grant Support | |
ID/Acronym/Agency:
|
1R01GM081655-01A2/GM/NIGMS NIH HHS; R01 GM081655-01A2/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Infective Agents; 0/Antimicrobial Cationic Peptides; 0/DNA-Binding Proteins; 0/Lipid Bilayers; 0/Peptides, Cyclic; 0/Polymers; 118231-04-2/tachyplesin peptide, Tachypleus tridentatus |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Photodissociation of acetaldehyde and the absolute photoionization cross section of HCO.
Next Document: Algal Pheromone Biosynthesis: Stereochemical Analysis and Mechanistic Implications in Gametes of Ect...