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Oridonin inhibits cell growth by induction of apoptosis on human hepatocelluar carcinoma BEL-7402 cells.
MedLine Citation:
PMID:  16638644     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The present study was undertaken to investigate the mechanisms of oridonin induced apoptosis on human hepatocellular carcinoma BEL-7402 cells. BEL-7402 cells in culture medium in vitro were given different concentrations of oridonin. Cell proliferation was measured by MTT assay and [(3)H]-thymidine uptake, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by Hoechst 33258 staining and DNA fragmentation. Caspase-3 as well as Bcl-2 and Bax expressions were detected by Western blotting. The results revealed that oridonin could inhibit the growth of BEL-7402 cells and cause apoptosis significantly, the suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis were observed very clearly by Hoechst 33258 staining as well as DNA fragmentation analysis after the cells exposed to oridonin for 60h; Western blotting showed cleavage of the caspase-3 zymogen protein (32-kDa) with the appearance of its 20-kDa subunit; Along with the apoptotic process Bcl-2 expression was down-regulated and Bax expression up-regulated concurrently observed by Western blotting. We therefore conclude that oridonin can inhibit cell growth by induction of apoptosis in BEL-7402 cells via activation of caspase-3 as well as down-regulation of Bcl-2 and up-regulation of Bax expression. The results indicate that oridonin may be an important potential anti-hepatocellular carcinoma reagent.
Authors:
Jun-Feng Zhang; Jia-Jun Liu; Pei-Qing Liu; Dong-Jun Lin; Xu-Dong Li; Gui-Hua Chen
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Publication Detail:
Type:  Journal Article     Date:  2006-04-25
Journal Detail:
Title:  Hepatology research : the official journal of the Japan Society of Hepatology     Volume:  35     ISSN:  1386-6346     ISO Abbreviation:  Hepatol. Res.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-08-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9711801     Medline TA:  Hepatol Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  104-10     Citation Subset:  -    
Affiliation:
Department of Liver Transplantation, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, PR China.
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