| Oridonin inhibits cell growth by induction of apoptosis on human hepatocelluar carcinoma BEL-7402 cells. | |
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MedLine Citation:
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PMID: 16638644 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The present study was undertaken to investigate the mechanisms of oridonin induced apoptosis on human hepatocellular carcinoma BEL-7402 cells. BEL-7402 cells in culture medium in vitro were given different concentrations of oridonin. Cell proliferation was measured by MTT assay and [(3)H]-thymidine uptake, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by Hoechst 33258 staining and DNA fragmentation. Caspase-3 as well as Bcl-2 and Bax expressions were detected by Western blotting. The results revealed that oridonin could inhibit the growth of BEL-7402 cells and cause apoptosis significantly, the suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis were observed very clearly by Hoechst 33258 staining as well as DNA fragmentation analysis after the cells exposed to oridonin for 60h; Western blotting showed cleavage of the caspase-3 zymogen protein (32-kDa) with the appearance of its 20-kDa subunit; Along with the apoptotic process Bcl-2 expression was down-regulated and Bax expression up-regulated concurrently observed by Western blotting. We therefore conclude that oridonin can inhibit cell growth by induction of apoptosis in BEL-7402 cells via activation of caspase-3 as well as down-regulation of Bcl-2 and up-regulation of Bax expression. The results indicate that oridonin may be an important potential anti-hepatocellular carcinoma reagent. |
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Authors:
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Jun-Feng Zhang; Jia-Jun Liu; Pei-Qing Liu; Dong-Jun Lin; Xu-Dong Li; Gui-Hua Chen |
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Publication Detail:
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Type: Journal Article Date: 2006-04-25 |
Journal Detail:
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Title: Hepatology research : the official journal of the Japan Society of Hepatology Volume: 35 ISSN: 1386-6346 ISO Abbreviation: Hepatol. Res. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-08-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9711801 Medline TA: Hepatol Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 104-10 Citation Subset: - |
Affiliation:
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Department of Liver Transplantation, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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