Document Detail

Organotypic cultures of the rat anterior pituitary: morphology, physiology and cell-to-cell communication.
MedLine Citation:
PMID:  9196288     Owner:  NLM     Status:  MEDLINE    
Organotypic cultures, prepared from young rats, were used to investigate the neuroendocrine properties of anterior pituitary cells. Pituitary cells maintained the features of endocrine cells, up to 7 weeks in vitro. Secretory granules could be seen with electron microscopy, and cells contained immunocytochemically detectable levels of adenohypophyseal hormones. Significant levels of prolactin (PRL), growth hormone and luteinizing hormone were present in the culture media after several weeks in vitro and PRL release could be modulated by dopaminergic agonists or forskolin. The electrophysiological properties of pituitary cells were investigated with both intracellular and patch-clamp recordings after 2 to 7 weeks in vitro. Cellular resting membrane potentials were approximately -50 mV, and spontaneous or depolarization-induced action potentials were found in approximately 50% of cells. Records of voltage-dependent outward membrane currents showed that cells expressed functional voltage-gated channels. Cells remained responsive to hypothalamic neuropeptides, as shown by the outward membrane current triggered by thyrotropin-releasing hormone. Intracellularly injected Lucifer Yellow readily diffused between neighboring cells, suggesting the presence of gap junctions. These data confirm the viability of organotypic cultures of the anterior pituitary gland, and demonstrate that the characteristic properties of this excitable endocrine tissue are conserved. This neuroendocrine preparation is suitable for studying the mechanisms regulating cell-to-cell communication under conditions resembling the in vivo tissue organization.
N C Guérineau; R A McKinney; D Debanne; P Mollard; B H Gähwiler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  73     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-08-25     Completed Date:  1997-08-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  169-76     Citation Subset:  IM    
Brain Research Institute, University of Zurich, Switzerland.
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MeSH Terms
Cell Communication*
Fluorescent Antibody Technique
Microscopy, Confocal
Microscopy, Electron
Organ Culture Techniques
Patch-Clamp Techniques
Pituitary Gland, Anterior / anatomy & histology*,  cytology,  physiology*
Thyrotropin-Releasing Hormone / pharmacology
Reg. No./Substance:
24305-27-9/Thyrotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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