Document Detail


Organotypic cultures of the rat anterior pituitary: morphology, physiology and cell-to-cell communication.
MedLine Citation:
PMID:  9196288     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Organotypic cultures, prepared from young rats, were used to investigate the neuroendocrine properties of anterior pituitary cells. Pituitary cells maintained the features of endocrine cells, up to 7 weeks in vitro. Secretory granules could be seen with electron microscopy, and cells contained immunocytochemically detectable levels of adenohypophyseal hormones. Significant levels of prolactin (PRL), growth hormone and luteinizing hormone were present in the culture media after several weeks in vitro and PRL release could be modulated by dopaminergic agonists or forskolin. The electrophysiological properties of pituitary cells were investigated with both intracellular and patch-clamp recordings after 2 to 7 weeks in vitro. Cellular resting membrane potentials were approximately -50 mV, and spontaneous or depolarization-induced action potentials were found in approximately 50% of cells. Records of voltage-dependent outward membrane currents showed that cells expressed functional voltage-gated channels. Cells remained responsive to hypothalamic neuropeptides, as shown by the outward membrane current triggered by thyrotropin-releasing hormone. Intracellularly injected Lucifer Yellow readily diffused between neighboring cells, suggesting the presence of gap junctions. These data confirm the viability of organotypic cultures of the anterior pituitary gland, and demonstrate that the characteristic properties of this excitable endocrine tissue are conserved. This neuroendocrine preparation is suitable for studying the mechanisms regulating cell-to-cell communication under conditions resembling the in vivo tissue organization.
Authors:
N C Guérineau; R A McKinney; D Debanne; P Mollard; B H Gähwiler
Related Documents :
7418658 - The effects of estrogen on prolactin cells of the male rat pituitary. an immunocytologi...
22395148 - The chemopreventive agent ursodeoxycholic acid inhibits proliferation of colon carcinom...
10746658 - Localization of leptin receptor-like immunoreactivity in the corticotropes, somatotrope...
7964308 - Effect of pituitary adenylate cyclase-activating polypeptide 38 on growth hormone and p...
15619228 - Cranial neural crest contributes to the bony skull vault in adult xenopus laevis: insig...
16153248 - Signalization and cytoskeleton activity through myosin ib during the early steps of pha...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  73     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-08-25     Completed Date:  1997-08-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  169-76     Citation Subset:  IM    
Affiliation:
Brain Research Institute, University of Zurich, Switzerland. guerinea@u469.montp.inserm.fr
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Communication*
Electrophysiology
Female
Fluorescent Antibody Technique
Male
Microscopy, Confocal
Microscopy, Electron
Organ Culture Techniques
Patch-Clamp Techniques
Pituitary Gland, Anterior / anatomy & histology*,  cytology,  physiology*
Rats
Thyrotropin-Releasing Hormone / pharmacology
Chemical
Reg. No./Substance:
24305-27-9/Thyrotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A quantitative computer-assisted morphometric analysis of stimulation-induced injury to myelinated f...
Next Document:  Long term chronic recordings from peripheral sensory fibers using a sieve electrode array.