| Organophosphorus pesticides induce apoptosis in human NK cells. | |
| | |
MedLine Citation:
|
PMID: 17681413 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We found previously that organophosphorus pesticides (OPs) significantly inhibited natural killer (NK) activity. To explore the mechanism of OP-induced inhibition of NK activity, we investigated whether OPs can induce apoptosis in NK cells in the present study. We used NK-92CI and NK-92MI cells, which are interleukin-2 independent human NK cell lines. NK-92CI and/or NK-92MI were treated with dichlorvos (DDVP) or chlorpyrifos (CP) at 0-100 ppm for 1-72 h at 37 degrees C in vitro. Apoptosis induced by DDVP and CP was determined by FITC-Annexin V staining and the intracellular level of active caspase-3 was analysed by flow cytometry. We found that DDVP and CP significantly induced apoptosis in NK-92CI and NK-92MI cells in a dose- and time-dependent manner. DDVP also induced an increase of intracellular active caspase-3 in NK-92CI in a dose- and time-dependent manner, and a caspase-3 inhibitor, Z-DEVD-FMK, significantly inhibited DDVP-induced apoptosis, suggesting that this apoptosis is partially mediated by activation of intracellular caspase-3. The pattern of apoptosis induced by CP differed from that induced by DDVP. CP showed a faster response than DDVP at higher doses; whereas, DDVP showed a slower, but stronger apoptosis-inducing ability than CP at lower doses. Moreover, the response to OPs differed between NK-92CI and NK-92MI cells, and NK-92CI was more sensitive to OPs than NK-92MI. This is similar to the inhibition of NK activity induced by DDVP, in which NK-92CI was more easily inhibited by DDVP than NK-92MI. Taken together, these findings suggest that OP-induced inhibition of NK activity may be at least partially mediated by OP-induced apoptosis in NK. |
| | |
Authors:
|
Qing Li; Maiko Kobayashi; Tomoyuki Kawada |
Related Documents
:
|
21846723 - Hemolysin induces toll-like receptor (tlr) independent apoptosis but multiple tlr assoc... 22433373 - Can microbial superantigens influence atopic dermatitis flares? 22078373 - Dendritic cell lineage commitment is instructed by distinct cytokine signals. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-07-05 |
Journal Detail:
|
Title: Toxicology Volume: 239 ISSN: 0300-483X ISO Abbreviation: Toxicology Publication Date: 2007 Sep |
Date Detail:
|
Created Date: 2007-08-27 Completed Date: 2007-10-16 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0361055 Medline TA: Toxicology Country: Ireland |
Other Details:
|
Languages: eng Pagination: 89-95 Citation Subset: IM |
Affiliation:
|
Department of Hygiene and Public Health, Nippon Medical School, 1-1-5 Sendagi, Tokyo, Japan. qing-li@nms.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects* Caspase 3 / metabolism Cell Line Chlorpyrifos / toxicity* Cysteine Proteinase Inhibitors / pharmacology Dichlorvos / toxicity* Dose-Response Relationship, Drug Drug Antagonism Humans Insecticides / toxicity* Killer Cells, Natural / drug effects*, pathology Oligopeptides / pharmacology Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Cysteine Proteinase Inhibitors; 0/Insecticides; 0/Oligopeptides; 0/benzoylcarbonyl-aspartyl-glutamyl-valyl-aspartyl-fluoromethyl ketone; 2921-88-2/Chlorpyrifos; 62-73-7/Dichlorvos; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evaluation of some pollutant levels in bitter orange trees: implications for human health.
Next Document: Spermatogenesis in aged rats after prenatal 3,3',4,4',5-pentachlorobiphenyl exposure.